BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing

OBJECTIVE: We aimed to investigate the prevalence and type of BRAF V600 mutations and the associations with clinicopathological variables in primary skin nodular melanoma cases in Yogyakarta and Central Java, Indonesia using pyrosequencing. RESULTS: BRAF V600 mutations of the V600E type were found i...

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Autores principales: Rinonce, Hanggoro Tri, Aji, Rovi Panji Mustiko, Hayati, Ni’mah, Pudjohartono, Maria Fransiska, Kameswari, Bidari, Anwar, Sumadi Lukman, Irianiwati
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081676/
https://www.ncbi.nlm.nih.gov/pubmed/32188503
http://dx.doi.org/10.1186/s13104-020-05000-w
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author Rinonce, Hanggoro Tri
Aji, Rovi Panji Mustiko
Hayati, Ni’mah
Pudjohartono, Maria Fransiska
Kameswari, Bidari
Anwar, Sumadi Lukman
Irianiwati
author_facet Rinonce, Hanggoro Tri
Aji, Rovi Panji Mustiko
Hayati, Ni’mah
Pudjohartono, Maria Fransiska
Kameswari, Bidari
Anwar, Sumadi Lukman
Irianiwati
author_sort Rinonce, Hanggoro Tri
collection PubMed
description OBJECTIVE: We aimed to investigate the prevalence and type of BRAF V600 mutations and the associations with clinicopathological variables in primary skin nodular melanoma cases in Yogyakarta and Central Java, Indonesia using pyrosequencing. RESULTS: BRAF V600 mutations of the V600E type were found in 21 (53.85%) specimens. The variant allele frequencies (VAFs) ranged from 5.07 to 94.70%, averaging 29.05%. However, most cases had low VAFs, with 13 (61.9%) specimens below 20% and 4 (19.05%) below 10%.
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spelling pubmed-70816762020-03-23 BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing Rinonce, Hanggoro Tri Aji, Rovi Panji Mustiko Hayati, Ni’mah Pudjohartono, Maria Fransiska Kameswari, Bidari Anwar, Sumadi Lukman Irianiwati BMC Res Notes Research Note OBJECTIVE: We aimed to investigate the prevalence and type of BRAF V600 mutations and the associations with clinicopathological variables in primary skin nodular melanoma cases in Yogyakarta and Central Java, Indonesia using pyrosequencing. RESULTS: BRAF V600 mutations of the V600E type were found in 21 (53.85%) specimens. The variant allele frequencies (VAFs) ranged from 5.07 to 94.70%, averaging 29.05%. However, most cases had low VAFs, with 13 (61.9%) specimens below 20% and 4 (19.05%) below 10%. BioMed Central 2020-03-18 /pmc/articles/PMC7081676/ /pubmed/32188503 http://dx.doi.org/10.1186/s13104-020-05000-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Rinonce, Hanggoro Tri
Aji, Rovi Panji Mustiko
Hayati, Ni’mah
Pudjohartono, Maria Fransiska
Kameswari, Bidari
Anwar, Sumadi Lukman
Irianiwati
BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing
title BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing
title_full BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing
title_fullStr BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing
title_full_unstemmed BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing
title_short BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing
title_sort braf v600 mutation profiling in primary skin nodular melanoma in indonesia: an analysis using high resolution pyrosequencing
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081676/
https://www.ncbi.nlm.nih.gov/pubmed/32188503
http://dx.doi.org/10.1186/s13104-020-05000-w
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