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Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model

Thousands of people are affected by central nervous system (CNS) dysfunctions each year, with stroke and spinal cord injury (SCI) being the most frequent causes. Although there is some evidence of partial CNS self-repair (via migration of neural stem cells to the injury zone and adult neurogenesis),...

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Autor principal: Demircan, Turan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cureus 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081738/
https://www.ncbi.nlm.nih.gov/pubmed/32211250
http://dx.doi.org/10.7759/cureus.7014
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author Demircan, Turan
author_facet Demircan, Turan
author_sort Demircan, Turan
collection PubMed
description Thousands of people are affected by central nervous system (CNS) dysfunctions each year, with stroke and spinal cord injury (SCI) being the most frequent causes. Although there is some evidence of partial CNS self-repair (via migration of neural stem cells to the injury zone and adult neurogenesis), due to restricted regeneration capacity in mammals, acute or chronic spinal cord injuries cannot be repaired completely. Therefore, to expand the availability of treatment options for SCI, research on highly regenerative animals has become essential. Among vertebrates, axolotl, a salamander species, has been emerging as a powerful model to explore the molecular mechanisms of regeneration due to its exceptional regenerative capacity. In this study, gene expression modulation for regenerative-capable neotenic axolotl during spinal cord regeneration has been investigated. Next-generation sequencing was applied for the collected regeneration samples at zero and seven days post-amputation (dpa). The data obtained from the analyzed samples revealed 363 genes differentially expressed, mostly downregulated, between zero dpa and seven dpa. The extracellular matrix, cell-cell adhesion, and immune system-related processes and pathways were enriched by gene ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Based on these data, we conclude that the downregulation of immune system-related biological processes is crucial for spinal cord regeneration.
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spelling pubmed-70817382020-03-24 Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model Demircan, Turan Cureus Genetics Thousands of people are affected by central nervous system (CNS) dysfunctions each year, with stroke and spinal cord injury (SCI) being the most frequent causes. Although there is some evidence of partial CNS self-repair (via migration of neural stem cells to the injury zone and adult neurogenesis), due to restricted regeneration capacity in mammals, acute or chronic spinal cord injuries cannot be repaired completely. Therefore, to expand the availability of treatment options for SCI, research on highly regenerative animals has become essential. Among vertebrates, axolotl, a salamander species, has been emerging as a powerful model to explore the molecular mechanisms of regeneration due to its exceptional regenerative capacity. In this study, gene expression modulation for regenerative-capable neotenic axolotl during spinal cord regeneration has been investigated. Next-generation sequencing was applied for the collected regeneration samples at zero and seven days post-amputation (dpa). The data obtained from the analyzed samples revealed 363 genes differentially expressed, mostly downregulated, between zero dpa and seven dpa. The extracellular matrix, cell-cell adhesion, and immune system-related processes and pathways were enriched by gene ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Based on these data, we conclude that the downregulation of immune system-related biological processes is crucial for spinal cord regeneration. Cureus 2020-02-16 /pmc/articles/PMC7081738/ /pubmed/32211250 http://dx.doi.org/10.7759/cureus.7014 Text en Copyright © 2020, Demircan et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Genetics
Demircan, Turan
Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model
title Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model
title_full Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model
title_fullStr Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model
title_full_unstemmed Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model
title_short Dissecting the Molecular Signature of Spinal Cord Regeneration in the Axolotl Model
title_sort dissecting the molecular signature of spinal cord regeneration in the axolotl model
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7081738/
https://www.ncbi.nlm.nih.gov/pubmed/32211250
http://dx.doi.org/10.7759/cureus.7014
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