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Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors
High energy laser, particularly 532 nm, is widely used in defense and medical applications and there is need to address its occupational safety. Thermal and non-thermal effects of 532 nm high energy laser on skin are cause of concern. This study indicates impact of 532 nm laser on rat skin and first...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082019/ https://www.ncbi.nlm.nih.gov/pubmed/32191734 http://dx.doi.org/10.1371/journal.pone.0230175 |
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author | Tulsawani, Rajkumar Sharma, Purva Sethy, Niroj Kumar Kumari, Pooja Ganju, Lilly Prakash, Satya Chouhan, Satish |
author_facet | Tulsawani, Rajkumar Sharma, Purva Sethy, Niroj Kumar Kumari, Pooja Ganju, Lilly Prakash, Satya Chouhan, Satish |
author_sort | Tulsawani, Rajkumar |
collection | PubMed |
description | High energy laser, particularly 532 nm, is widely used in defense and medical applications and there is need to address its occupational safety. Thermal and non-thermal effects of 532 nm high energy laser on skin are cause of concern. This study indicates impact of 532 nm laser on rat skin and first of its kind of attempt to understand transcriptional activation of genes as an early response following laser exposure. Skin of experimental rats were exposed to 532 nm radiance at 0.1, 0.25 and 0.50 W/cm(2) for 10 sec. Thermographic changes of skin exposed to 532 nm laser exhibited increased Tmax temperature in radiance dependent manner. After thermal imaging, skin of experimental rats was collected 1 h post laser exposure for studying differential gene expression. The skin exposed to lower power density (0.1 W/cm(2)) did not show significant changes in expression of gene pathways studied. At moderate radiance (0.25 W/cm(2)), predominantly canonical wnt/B-catenin pathway genes notch1, axin2, ccdn1, wnt5a and redox homeostasis genes; txn1, nqo1 and txnrd1 were expressed. At higher radiance (0.5 W/cm(2)), significant repression of genes related to wound healing process particularly notch/wnt pathway viz. hes5, wnt1, wn3b with higher expression of dab2 was recorded. The data obtained from these studies would help in drawing safety limits for skin exposure to 532 nm laser. Further, genes expressed at moderate and high level of radiance exposure to skin were distinct and differential and provide new avenue to configure pathway to counteract laser induced delay in tissue injury and hair follicular damage. |
format | Online Article Text |
id | pubmed-7082019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-70820192020-03-24 Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors Tulsawani, Rajkumar Sharma, Purva Sethy, Niroj Kumar Kumari, Pooja Ganju, Lilly Prakash, Satya Chouhan, Satish PLoS One Research Article High energy laser, particularly 532 nm, is widely used in defense and medical applications and there is need to address its occupational safety. Thermal and non-thermal effects of 532 nm high energy laser on skin are cause of concern. This study indicates impact of 532 nm laser on rat skin and first of its kind of attempt to understand transcriptional activation of genes as an early response following laser exposure. Skin of experimental rats were exposed to 532 nm radiance at 0.1, 0.25 and 0.50 W/cm(2) for 10 sec. Thermographic changes of skin exposed to 532 nm laser exhibited increased Tmax temperature in radiance dependent manner. After thermal imaging, skin of experimental rats was collected 1 h post laser exposure for studying differential gene expression. The skin exposed to lower power density (0.1 W/cm(2)) did not show significant changes in expression of gene pathways studied. At moderate radiance (0.25 W/cm(2)), predominantly canonical wnt/B-catenin pathway genes notch1, axin2, ccdn1, wnt5a and redox homeostasis genes; txn1, nqo1 and txnrd1 were expressed. At higher radiance (0.5 W/cm(2)), significant repression of genes related to wound healing process particularly notch/wnt pathway viz. hes5, wnt1, wn3b with higher expression of dab2 was recorded. The data obtained from these studies would help in drawing safety limits for skin exposure to 532 nm laser. Further, genes expressed at moderate and high level of radiance exposure to skin were distinct and differential and provide new avenue to configure pathway to counteract laser induced delay in tissue injury and hair follicular damage. Public Library of Science 2020-03-19 /pmc/articles/PMC7082019/ /pubmed/32191734 http://dx.doi.org/10.1371/journal.pone.0230175 Text en © 2020 Tulsawani et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Tulsawani, Rajkumar Sharma, Purva Sethy, Niroj Kumar Kumari, Pooja Ganju, Lilly Prakash, Satya Chouhan, Satish Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors |
title | Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors |
title_full | Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors |
title_fullStr | Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors |
title_full_unstemmed | Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors |
title_short | Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors |
title_sort | acute exposure of 532 nm laser differentially regulates skin tissue transcription factors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082019/ https://www.ncbi.nlm.nih.gov/pubmed/32191734 http://dx.doi.org/10.1371/journal.pone.0230175 |
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