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Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function
A recent genome-wide screen identified ~300 essential or growth-supporting genes in the dental caries pathogen Streptococcus mutans. To be able to study these genes, we built a CRISPR interference tool around the Cas9 nuclease (Cas9(Smu)) encoded in the S. mutans UA159 genome. Using a xylose-inducib...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082069/ https://www.ncbi.nlm.nih.gov/pubmed/32150575 http://dx.doi.org/10.1371/journal.ppat.1008344 |