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Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function

A recent genome-wide screen identified ~300 essential or growth-supporting genes in the dental caries pathogen Streptococcus mutans. To be able to study these genes, we built a CRISPR interference tool around the Cas9 nuclease (Cas9(Smu)) encoded in the S. mutans UA159 genome. Using a xylose-inducib...

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Detalles Bibliográficos
Autores principales:	Shields, Robert C., Walker, Alejandro R., Maricic, Natalie, Chakraborty, Brinta, Underhill, Simon A. M., Burne, Robert A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082069/ https://www.ncbi.nlm.nih.gov/pubmed/32150575 http://dx.doi.org/10.1371/journal.ppat.1008344

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