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New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses

Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S(0) and [Formula: see text] metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectros...

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Autores principales: Camacho, David, Frazao, Rodolfo, Fouillen, Aurélien, Nanci, Antonio, Lang, B. Franz, Apte, Simon C., Baron, Christian, Warren, Lesley A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082400/
https://www.ncbi.nlm.nih.gov/pubmed/32231653
http://dx.doi.org/10.3389/fmicb.2020.00411
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author Camacho, David
Frazao, Rodolfo
Fouillen, Aurélien
Nanci, Antonio
Lang, B. Franz
Apte, Simon C.
Baron, Christian
Warren, Lesley A.
author_facet Camacho, David
Frazao, Rodolfo
Fouillen, Aurélien
Nanci, Antonio
Lang, B. Franz
Apte, Simon C.
Baron, Christian
Warren, Lesley A.
author_sort Camacho, David
collection PubMed
description Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S(0) and [Formula: see text] metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectroscopy. The A. thiooxidans [Formula: see text] metabolism model involves the conversion of [Formula: see text] to [Formula: see text] , S(0) and [Formula: see text] mediated by the sulfur oxidase complex (Sox), tetrathionate hydrolase (TetH), sulfide quinone reductase (Sqr), and heterodisulfate reductase (Hdr) proteins. These same proteins, with the addition of rhodanese (Rhd), were identified to convert S(0) to [Formula: see text] , [Formula: see text] and polythionates in the A. thiooxidans S(0) metabolism model. Our combined results shed light onto the important role specifically of TetH in [Formula: see text] metabolism. Also, we show that activity of Hdr proteins rather than Sdo are likely associated with S(0) oxidation. Finally, our data suggest that formation of intracellular [Formula: see text] is a critical step in S(0) metabolism, and that recycling of internally generated [Formula: see text] occurs, through comproportionating reactions that result in [Formula: see text]. Electron microscopy and spectroscopy confirmed intracellular production and storage of S(0) during growth on both S(0) and [Formula: see text] substrates.
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spelling pubmed-70824002020-03-30 New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses Camacho, David Frazao, Rodolfo Fouillen, Aurélien Nanci, Antonio Lang, B. Franz Apte, Simon C. Baron, Christian Warren, Lesley A. Front Microbiol Microbiology Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S(0) and [Formula: see text] metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectroscopy. The A. thiooxidans [Formula: see text] metabolism model involves the conversion of [Formula: see text] to [Formula: see text] , S(0) and [Formula: see text] mediated by the sulfur oxidase complex (Sox), tetrathionate hydrolase (TetH), sulfide quinone reductase (Sqr), and heterodisulfate reductase (Hdr) proteins. These same proteins, with the addition of rhodanese (Rhd), were identified to convert S(0) to [Formula: see text] , [Formula: see text] and polythionates in the A. thiooxidans S(0) metabolism model. Our combined results shed light onto the important role specifically of TetH in [Formula: see text] metabolism. Also, we show that activity of Hdr proteins rather than Sdo are likely associated with S(0) oxidation. Finally, our data suggest that formation of intracellular [Formula: see text] is a critical step in S(0) metabolism, and that recycling of internally generated [Formula: see text] occurs, through comproportionating reactions that result in [Formula: see text]. Electron microscopy and spectroscopy confirmed intracellular production and storage of S(0) during growth on both S(0) and [Formula: see text] substrates. Frontiers Media S.A. 2020-03-13 /pmc/articles/PMC7082400/ /pubmed/32231653 http://dx.doi.org/10.3389/fmicb.2020.00411 Text en Copyright © 2020 Camacho, Frazao, Fouillen, Nanci, Lang, Apte, Baron and Warren. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Camacho, David
Frazao, Rodolfo
Fouillen, Aurélien
Nanci, Antonio
Lang, B. Franz
Apte, Simon C.
Baron, Christian
Warren, Lesley A.
New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses
title New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses
title_full New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses
title_fullStr New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses
title_full_unstemmed New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses
title_short New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses
title_sort new insights into acidithiobacillus thiooxidans sulfur metabolism through coupled gene expression, solution chemistry, microscopy, and spectroscopy analyses
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082400/
https://www.ncbi.nlm.nih.gov/pubmed/32231653
http://dx.doi.org/10.3389/fmicb.2020.00411
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