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New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses
Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S(0) and [Formula: see text] metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectros...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082400/ https://www.ncbi.nlm.nih.gov/pubmed/32231653 http://dx.doi.org/10.3389/fmicb.2020.00411 |
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author | Camacho, David Frazao, Rodolfo Fouillen, Aurélien Nanci, Antonio Lang, B. Franz Apte, Simon C. Baron, Christian Warren, Lesley A. |
author_facet | Camacho, David Frazao, Rodolfo Fouillen, Aurélien Nanci, Antonio Lang, B. Franz Apte, Simon C. Baron, Christian Warren, Lesley A. |
author_sort | Camacho, David |
collection | PubMed |
description | Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S(0) and [Formula: see text] metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectroscopy. The A. thiooxidans [Formula: see text] metabolism model involves the conversion of [Formula: see text] to [Formula: see text] , S(0) and [Formula: see text] mediated by the sulfur oxidase complex (Sox), tetrathionate hydrolase (TetH), sulfide quinone reductase (Sqr), and heterodisulfate reductase (Hdr) proteins. These same proteins, with the addition of rhodanese (Rhd), were identified to convert S(0) to [Formula: see text] , [Formula: see text] and polythionates in the A. thiooxidans S(0) metabolism model. Our combined results shed light onto the important role specifically of TetH in [Formula: see text] metabolism. Also, we show that activity of Hdr proteins rather than Sdo are likely associated with S(0) oxidation. Finally, our data suggest that formation of intracellular [Formula: see text] is a critical step in S(0) metabolism, and that recycling of internally generated [Formula: see text] occurs, through comproportionating reactions that result in [Formula: see text]. Electron microscopy and spectroscopy confirmed intracellular production and storage of S(0) during growth on both S(0) and [Formula: see text] substrates. |
format | Online Article Text |
id | pubmed-7082400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70824002020-03-30 New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses Camacho, David Frazao, Rodolfo Fouillen, Aurélien Nanci, Antonio Lang, B. Franz Apte, Simon C. Baron, Christian Warren, Lesley A. Front Microbiol Microbiology Here, we experimentally expand understanding of the reactions and enzymes involved in Acidithiobacillus thiooxidans ATCC 19377 S(0) and [Formula: see text] metabolism by developing models that integrate gene expression analyzed by RNA-Seq, solution sulfur speciation, electron microscopy and spectroscopy. The A. thiooxidans [Formula: see text] metabolism model involves the conversion of [Formula: see text] to [Formula: see text] , S(0) and [Formula: see text] mediated by the sulfur oxidase complex (Sox), tetrathionate hydrolase (TetH), sulfide quinone reductase (Sqr), and heterodisulfate reductase (Hdr) proteins. These same proteins, with the addition of rhodanese (Rhd), were identified to convert S(0) to [Formula: see text] , [Formula: see text] and polythionates in the A. thiooxidans S(0) metabolism model. Our combined results shed light onto the important role specifically of TetH in [Formula: see text] metabolism. Also, we show that activity of Hdr proteins rather than Sdo are likely associated with S(0) oxidation. Finally, our data suggest that formation of intracellular [Formula: see text] is a critical step in S(0) metabolism, and that recycling of internally generated [Formula: see text] occurs, through comproportionating reactions that result in [Formula: see text]. Electron microscopy and spectroscopy confirmed intracellular production and storage of S(0) during growth on both S(0) and [Formula: see text] substrates. Frontiers Media S.A. 2020-03-13 /pmc/articles/PMC7082400/ /pubmed/32231653 http://dx.doi.org/10.3389/fmicb.2020.00411 Text en Copyright © 2020 Camacho, Frazao, Fouillen, Nanci, Lang, Apte, Baron and Warren. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Camacho, David Frazao, Rodolfo Fouillen, Aurélien Nanci, Antonio Lang, B. Franz Apte, Simon C. Baron, Christian Warren, Lesley A. New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses |
title | New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses |
title_full | New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses |
title_fullStr | New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses |
title_full_unstemmed | New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses |
title_short | New Insights Into Acidithiobacillus thiooxidans Sulfur Metabolism Through Coupled Gene Expression, Solution Chemistry, Microscopy, and Spectroscopy Analyses |
title_sort | new insights into acidithiobacillus thiooxidans sulfur metabolism through coupled gene expression, solution chemistry, microscopy, and spectroscopy analyses |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082400/ https://www.ncbi.nlm.nih.gov/pubmed/32231653 http://dx.doi.org/10.3389/fmicb.2020.00411 |
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