A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry

In biomedical and preclinical research, the current standard method for measuring blood perfusion inside murine bone, radiolabeled microspheres, is a terminal procedure that cannot be used to monitor longitudinal perfusion changes. Laser Doppler flowmetry (LDF) can assess perfusion within the proxim...

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Detalles Bibliográficos
Autores principales: Hanne, Nicholas J., Easter, Elizabeth D., Stangeland-Molo, Sandra, Cole, Jacqueline H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Medicine and Dentistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082608/
https://www.ncbi.nlm.nih.gov/pubmed/32211303
http://dx.doi.org/10.1016/j.mex.2020.100814
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author Hanne, Nicholas J.
Easter, Elizabeth D.
Stangeland-Molo, Sandra
Cole, Jacqueline H.
author_facet Hanne, Nicholas J.
Easter, Elizabeth D.
Stangeland-Molo, Sandra
Cole, Jacqueline H.
author_sort Hanne, Nicholas J.
collection PubMed
description In biomedical and preclinical research, the current standard method for measuring blood perfusion inside murine bone, radiolabeled microspheres, is a terminal procedure that cannot be used to monitor longitudinal perfusion changes. Laser Doppler flowmetry (LDF) can assess perfusion within the proximal tibial metaphysis of mice in vivo • Unlike previous endpoint-only techniques, this modified LDF procedure can be performed weekly to monitor serial changes to intraosseous perfusion in the murine tibia; • The modified LDF technique utilizes a smaller, more localized incision to minimize invasiveness and speed recovery.
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spelling pubmed-70826082020-03-24 A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry Hanne, Nicholas J. Easter, Elizabeth D. Stangeland-Molo, Sandra Cole, Jacqueline H. MethodsX Medicine and Dentistry In biomedical and preclinical research, the current standard method for measuring blood perfusion inside murine bone, radiolabeled microspheres, is a terminal procedure that cannot be used to monitor longitudinal perfusion changes. Laser Doppler flowmetry (LDF) can assess perfusion within the proximal tibial metaphysis of mice in vivo • Unlike previous endpoint-only techniques, this modified LDF procedure can be performed weekly to monitor serial changes to intraosseous perfusion in the murine tibia; • The modified LDF technique utilizes a smaller, more localized incision to minimize invasiveness and speed recovery. Elsevier 2020-02-20 /pmc/articles/PMC7082608/ /pubmed/32211303 http://dx.doi.org/10.1016/j.mex.2020.100814 Text en © 2020 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Medicine and Dentistry
Hanne, Nicholas J.
Easter, Elizabeth D.
Stangeland-Molo, Sandra
Cole, Jacqueline H.
A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry
title A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry
title_full A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry
title_fullStr A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry
title_full_unstemmed A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry
title_short A minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser Doppler flowmetry
title_sort minimally invasive technique for serial intraosseous perfusion measurements in the murine tibia using laser doppler flowmetry
topic Medicine and Dentistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082608/
https://www.ncbi.nlm.nih.gov/pubmed/32211303
http://dx.doi.org/10.1016/j.mex.2020.100814
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