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Disparity of Cytochrome Utilization in Anodic and Cathodic Extracellular Electron Transfer Pathways of Geobacter sulfurreducens Biofilms
[Image: see text] Extracellular electron transfer (EET) in microorganisms is prevalent in nature and has been utilized in functional bioelectrochemical systems. EET of Geobacter sulfurreducens has been extensively studied and has been revealed to be facilitated through c-type cytochromes, which medi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082794/ https://www.ncbi.nlm.nih.gov/pubmed/32066233 http://dx.doi.org/10.1021/jacs.9b13077 |
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author | Heidary, Nina Kornienko, Nikolay Kalathil, Shafeer Fang, Xin Ly, Khoa H. Greer, Heather F. Reisner, Erwin |
author_facet | Heidary, Nina Kornienko, Nikolay Kalathil, Shafeer Fang, Xin Ly, Khoa H. Greer, Heather F. Reisner, Erwin |
author_sort | Heidary, Nina |
collection | PubMed |
description | [Image: see text] Extracellular electron transfer (EET) in microorganisms is prevalent in nature and has been utilized in functional bioelectrochemical systems. EET of Geobacter sulfurreducens has been extensively studied and has been revealed to be facilitated through c-type cytochromes, which mediate charge between the electrode and G. sulfurreducens in anodic mode. However, the EET pathway of cathodic conversion of fumarate to succinate is still under debate. Here, we apply a variety of analytical methods, including electrochemistry, UV–vis absorption and resonance Raman spectroscopy, quartz crystal microbalance with dissipation, and electron microscopy, to understand the involvement of cytochromes and other possible electron-mediating species in the switching between anodic and cathodic reaction modes. By switching the applied bias for a G. sulfurreducens biofilm coupled to investigating the quantity and function of cytochromes, as well as the emergence of Fe-containing particles on the cell membrane, we provide evidence of a diminished role of cytochromes in cathodic EET. This work sheds light on the mechanisms of G. sulfurreducens biofilm growth and suggests the possible existence of a nonheme, iron-involving EET process in cathodic mode. |
format | Online Article Text |
id | pubmed-7082794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-70827942020-03-23 Disparity of Cytochrome Utilization in Anodic and Cathodic Extracellular Electron Transfer Pathways of Geobacter sulfurreducens Biofilms Heidary, Nina Kornienko, Nikolay Kalathil, Shafeer Fang, Xin Ly, Khoa H. Greer, Heather F. Reisner, Erwin J Am Chem Soc [Image: see text] Extracellular electron transfer (EET) in microorganisms is prevalent in nature and has been utilized in functional bioelectrochemical systems. EET of Geobacter sulfurreducens has been extensively studied and has been revealed to be facilitated through c-type cytochromes, which mediate charge between the electrode and G. sulfurreducens in anodic mode. However, the EET pathway of cathodic conversion of fumarate to succinate is still under debate. Here, we apply a variety of analytical methods, including electrochemistry, UV–vis absorption and resonance Raman spectroscopy, quartz crystal microbalance with dissipation, and electron microscopy, to understand the involvement of cytochromes and other possible electron-mediating species in the switching between anodic and cathodic reaction modes. By switching the applied bias for a G. sulfurreducens biofilm coupled to investigating the quantity and function of cytochromes, as well as the emergence of Fe-containing particles on the cell membrane, we provide evidence of a diminished role of cytochromes in cathodic EET. This work sheds light on the mechanisms of G. sulfurreducens biofilm growth and suggests the possible existence of a nonheme, iron-involving EET process in cathodic mode. American Chemical Society 2020-02-17 2020-03-18 /pmc/articles/PMC7082794/ /pubmed/32066233 http://dx.doi.org/10.1021/jacs.9b13077 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Heidary, Nina Kornienko, Nikolay Kalathil, Shafeer Fang, Xin Ly, Khoa H. Greer, Heather F. Reisner, Erwin Disparity of Cytochrome Utilization in Anodic and Cathodic Extracellular Electron Transfer Pathways of Geobacter sulfurreducens Biofilms |
title | Disparity
of Cytochrome Utilization in Anodic and
Cathodic Extracellular Electron Transfer Pathways of Geobacter
sulfurreducens Biofilms |
title_full | Disparity
of Cytochrome Utilization in Anodic and
Cathodic Extracellular Electron Transfer Pathways of Geobacter
sulfurreducens Biofilms |
title_fullStr | Disparity
of Cytochrome Utilization in Anodic and
Cathodic Extracellular Electron Transfer Pathways of Geobacter
sulfurreducens Biofilms |
title_full_unstemmed | Disparity
of Cytochrome Utilization in Anodic and
Cathodic Extracellular Electron Transfer Pathways of Geobacter
sulfurreducens Biofilms |
title_short | Disparity
of Cytochrome Utilization in Anodic and
Cathodic Extracellular Electron Transfer Pathways of Geobacter
sulfurreducens Biofilms |
title_sort | disparity
of cytochrome utilization in anodic and
cathodic extracellular electron transfer pathways of geobacter
sulfurreducens biofilms |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082794/ https://www.ncbi.nlm.nih.gov/pubmed/32066233 http://dx.doi.org/10.1021/jacs.9b13077 |
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