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Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin
Brush border membrane vesicles (BBMVs) were prepared from the 2(nd) instar larvae of Helicoverpa armigera. Binding of the activated Cry1Ac of Bacillus thuringiensis (Bt) toxin was shown by immunoblot. A 120-kDa protein was identified as a receptor for the Cry1Ac type δ-endotoxin. The aminopeptidase-...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2001
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082820/ https://www.ncbi.nlm.nih.gov/pubmed/11683359 http://dx.doi.org/10.1007/s002840010297 |
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author | Ingle, Sanjay S. Trivedi, Nidhi Prasad, Rakesh Kuruvilla, Jes Rao, Koteshwara Kukatla Chhatpar, H.S. |
author_facet | Ingle, Sanjay S. Trivedi, Nidhi Prasad, Rakesh Kuruvilla, Jes Rao, Koteshwara Kukatla Chhatpar, H.S. |
author_sort | Ingle, Sanjay S. |
collection | PubMed |
description | Brush border membrane vesicles (BBMVs) were prepared from the 2(nd) instar larvae of Helicoverpa armigera. Binding of the activated Cry1Ac of Bacillus thuringiensis (Bt) toxin was shown by immunoblot. A 120-kDa protein was identified as a receptor for the Cry1Ac type δ-endotoxin. The aminopeptidase-N activity of BBMVs was measured as the hydrolysis of L-leucine p-nitroanilide. The specific activity was 35 units/mg protein. The BBMV preparation also showed low level of alkaline phosphatase activity. Zn(++) chelating agents 2,2′-dipyridyl and 1,10-phenanthroline inhibited aminopeptidase activity at 10 mM concentration, indicating the presence of zinc-dependent aminopeptidase in the brush border of H. armigera. The aminopeptidase activity was increased with increasing concentration of δ-endotoxin. The purified 120-kDa binding protein was N-terminally sequenced. The first 10-amino-acid sequence showed 60–77% similarity with human cysteine-rich secretory protein-1 precursor, inhibin alpha chain precursor. Salmonella flagellar hook protein and yeast carboxypeptidase S. |
format | Online Article Text |
id | pubmed-7082820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2001 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-70828202020-03-23 Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin Ingle, Sanjay S. Trivedi, Nidhi Prasad, Rakesh Kuruvilla, Jes Rao, Koteshwara Kukatla Chhatpar, H.S. Curr Microbiol Article Brush border membrane vesicles (BBMVs) were prepared from the 2(nd) instar larvae of Helicoverpa armigera. Binding of the activated Cry1Ac of Bacillus thuringiensis (Bt) toxin was shown by immunoblot. A 120-kDa protein was identified as a receptor for the Cry1Ac type δ-endotoxin. The aminopeptidase-N activity of BBMVs was measured as the hydrolysis of L-leucine p-nitroanilide. The specific activity was 35 units/mg protein. The BBMV preparation also showed low level of alkaline phosphatase activity. Zn(++) chelating agents 2,2′-dipyridyl and 1,10-phenanthroline inhibited aminopeptidase activity at 10 mM concentration, indicating the presence of zinc-dependent aminopeptidase in the brush border of H. armigera. The aminopeptidase activity was increased with increasing concentration of δ-endotoxin. The purified 120-kDa binding protein was N-terminally sequenced. The first 10-amino-acid sequence showed 60–77% similarity with human cysteine-rich secretory protein-1 precursor, inhibin alpha chain precursor. Salmonella flagellar hook protein and yeast carboxypeptidase S. Springer-Verlag 2001 /pmc/articles/PMC7082820/ /pubmed/11683359 http://dx.doi.org/10.1007/s002840010297 Text en © Springer-Verlag New York Inc. 2001 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Article Ingle, Sanjay S. Trivedi, Nidhi Prasad, Rakesh Kuruvilla, Jes Rao, Koteshwara Kukatla Chhatpar, H.S. Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin |
title | Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin |
title_full | Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin |
title_fullStr | Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin |
title_full_unstemmed | Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin |
title_short | Aminopeptidase-N from the Helicoverpa armigera (Hubner) Brush Border Membrane Vesicles as a Receptor of Bacillus thuringiensis Cry1Ac δ-Endotoxin |
title_sort | aminopeptidase-n from the helicoverpa armigera (hubner) brush border membrane vesicles as a receptor of bacillus thuringiensis cry1ac δ-endotoxin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082820/ https://www.ncbi.nlm.nih.gov/pubmed/11683359 http://dx.doi.org/10.1007/s002840010297 |
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