Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer

BACKGROUND: Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. METHODS: The expression...

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Autores principales: Li, Ying, Liu, Yusong, Yao, Jie, Li, Rui, Fan, Xiaocheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082931/
https://www.ncbi.nlm.nih.gov/pubmed/32192507
http://dx.doi.org/10.1186/s13000-020-00946-8
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author Li, Ying
Liu, Yusong
Yao, Jie
Li, Rui
Fan, Xiaocheng
author_facet Li, Ying
Liu, Yusong
Yao, Jie
Li, Rui
Fan, Xiaocheng
author_sort Li, Ying
collection PubMed
description BACKGROUND: Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. METHODS: The expression of miR-484 in gastric cancer was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were employed to explore the prognostic significance of miR-484 in gastric cancer. The functional role of miR-484 in gastric cancer was determined by CCK-8 and Transwell assays. RESULTS: The results showed that miR-484 was significantly downregulated in gastric cancer tissues and cell lines. The downregulation of miR-484 was closely related to differentiation, lymph node metastasis, TNM stage, and poor prognosis. Cox regression analyses demonstrated that miR-484 was an independent prognosis indicator for gastric cancer patients. Additionally, the downregulation of miR-484 enhanced cell proliferation, migration, and invasion in gastric cancer cells. CONCLUSION: These data demonstrated that miR-484 can serve as a potential prognostic biomarker and therapeutic target for gastric cancer and it may be involved in the progression of gastric cancer.
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spelling pubmed-70829312020-03-23 Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer Li, Ying Liu, Yusong Yao, Jie Li, Rui Fan, Xiaocheng Diagn Pathol Research BACKGROUND: Gastric cancer is one of the most common cancers leading to high cancer mortality. MicroRNA-484 (miR-484) has been evaluated as a biomarker for various types of cancers. The subject of this study is to investigate the functional role of miR-484 in gastric cancer. METHODS: The expression of miR-484 in gastric cancer was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) assay. Kaplan-Meier survival and Cox regression analyses were employed to explore the prognostic significance of miR-484 in gastric cancer. The functional role of miR-484 in gastric cancer was determined by CCK-8 and Transwell assays. RESULTS: The results showed that miR-484 was significantly downregulated in gastric cancer tissues and cell lines. The downregulation of miR-484 was closely related to differentiation, lymph node metastasis, TNM stage, and poor prognosis. Cox regression analyses demonstrated that miR-484 was an independent prognosis indicator for gastric cancer patients. Additionally, the downregulation of miR-484 enhanced cell proliferation, migration, and invasion in gastric cancer cells. CONCLUSION: These data demonstrated that miR-484 can serve as a potential prognostic biomarker and therapeutic target for gastric cancer and it may be involved in the progression of gastric cancer. BioMed Central 2020-03-20 /pmc/articles/PMC7082931/ /pubmed/32192507 http://dx.doi.org/10.1186/s13000-020-00946-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Ying
Liu, Yusong
Yao, Jie
Li, Rui
Fan, Xiaocheng
Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer
title Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer
title_full Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer
title_fullStr Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer
title_full_unstemmed Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer
title_short Downregulation of miR-484 is associated with poor prognosis and tumor progression of gastric cancer
title_sort downregulation of mir-484 is associated with poor prognosis and tumor progression of gastric cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7082931/
https://www.ncbi.nlm.nih.gov/pubmed/32192507
http://dx.doi.org/10.1186/s13000-020-00946-8
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AT yaojie downregulationofmir484isassociatedwithpoorprognosisandtumorprogressionofgastriccancer
AT lirui downregulationofmir484isassociatedwithpoorprognosisandtumorprogressionofgastriccancer
AT fanxiaocheng downregulationofmir484isassociatedwithpoorprognosisandtumorprogressionofgastriccancer

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