Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri

Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamicin...

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Autores principales: Menanteau-Ledouble, Simon, Schachner, Oskar, Lawrence, Mark L., El-Matbouli, Mansour
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083013/
https://www.ncbi.nlm.nih.gov/pubmed/32197655
http://dx.doi.org/10.1186/s13567-020-00760-6
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author Menanteau-Ledouble, Simon
Schachner, Oskar
Lawrence, Mark L.
El-Matbouli, Mansour
author_facet Menanteau-Ledouble, Simon
Schachner, Oskar
Lawrence, Mark L.
El-Matbouli, Mansour
author_sort Menanteau-Ledouble, Simon
collection PubMed
description Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamicin assay to quantify the effect of silencing on the cells’ susceptibility to infection and found that silencing of 18 out of 20 genes significantly reduced the number of Y. ruckeri recovered. These findings improve our understanding of the infection process by Y. ruckeri and of the interactions between this bacterial pathogen and host cells.
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spelling pubmed-70830132020-03-23 Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri Menanteau-Ledouble, Simon Schachner, Oskar Lawrence, Mark L. El-Matbouli, Mansour Vet Res Short Report Yersinia ruckeri is a facultative intracellular enterobacterium mostly known as the causative agent of enteric redmouth disease in salmonid fish. In the present study, we applied RNA inhibition to silence twenty pre-selected genes on the genome of a fish cell line (CHSE-214) followed by a gentamicin assay to quantify the effect of silencing on the cells’ susceptibility to infection and found that silencing of 18 out of 20 genes significantly reduced the number of Y. ruckeri recovered. These findings improve our understanding of the infection process by Y. ruckeri and of the interactions between this bacterial pathogen and host cells. BioMed Central 2020-03-20 2020 /pmc/articles/PMC7083013/ /pubmed/32197655 http://dx.doi.org/10.1186/s13567-020-00760-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Menanteau-Ledouble, Simon
Schachner, Oskar
Lawrence, Mark L.
El-Matbouli, Mansour
Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
title Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
title_full Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
title_fullStr Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
title_full_unstemmed Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
title_short Effects of siRNA silencing on the susceptibility of the fish cell line CHSE-214 to Yersinia ruckeri
title_sort effects of sirna silencing on the susceptibility of the fish cell line chse-214 to yersinia ruckeri
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083013/
https://www.ncbi.nlm.nih.gov/pubmed/32197655
http://dx.doi.org/10.1186/s13567-020-00760-6
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