Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection

OBJECTIVE: The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress. RESULTS: 12 isolates were found over-expressing AcrAB-TolC efflux pump syste...

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Autores principales: Chetri, Shiela, Das, Bhaskar Jyoti, Bhowmik, Deepshikha, Chanda, Debadatta Dhar, Chakravarty, Atanu, Bhattacharjee, Amitabha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083032/
https://www.ncbi.nlm.nih.gov/pubmed/32192538
http://dx.doi.org/10.1186/s13104-020-04999-2
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author Chetri, Shiela
Das, Bhaskar Jyoti
Bhowmik, Deepshikha
Chanda, Debadatta Dhar
Chakravarty, Atanu
Bhattacharjee, Amitabha
author_facet Chetri, Shiela
Das, Bhaskar Jyoti
Bhowmik, Deepshikha
Chanda, Debadatta Dhar
Chakravarty, Atanu
Bhattacharjee, Amitabha
author_sort Chetri, Shiela
collection PubMed
description OBJECTIVE: The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress. RESULTS: 12 isolates were found over-expressing AcrAB-TolC efflux pump system and showed reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, over expression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems.
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spelling pubmed-70830322020-03-23 Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection Chetri, Shiela Das, Bhaskar Jyoti Bhowmik, Deepshikha Chanda, Debadatta Dhar Chakravarty, Atanu Bhattacharjee, Amitabha BMC Res Notes Research Note OBJECTIVE: The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress. RESULTS: 12 isolates were found over-expressing AcrAB-TolC efflux pump system and showed reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, over expression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems. BioMed Central 2020-03-19 /pmc/articles/PMC7083032/ /pubmed/32192538 http://dx.doi.org/10.1186/s13104-020-04999-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Chetri, Shiela
Das, Bhaskar Jyoti
Bhowmik, Deepshikha
Chanda, Debadatta Dhar
Chakravarty, Atanu
Bhattacharjee, Amitabha
Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection
title Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection
title_full Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection
title_fullStr Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection
title_full_unstemmed Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection
title_short Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection
title_sort transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within escherichia coli isolated from hospital acquired infection
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083032/
https://www.ncbi.nlm.nih.gov/pubmed/32192538
http://dx.doi.org/10.1186/s13104-020-04999-2
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