Chemical-defined medium supporting the expansion of human mesenchymal stem cells

OBJECTIVES: Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet. MATERIALS AND METHODS: Here, we developed the full chemical-defi...

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Autores principales: Xu, Jianyong, Lian, Wei, Chen, Jieting, Li, Wenlei, Li, Lingyun, Huang, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083066/
https://www.ncbi.nlm.nih.gov/pubmed/32192530
http://dx.doi.org/10.1186/s13287-020-01641-7
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author Xu, Jianyong
Lian, Wei
Chen, Jieting
Li, Wenlei
Li, Lingyun
Huang, Zhong
author_facet Xu, Jianyong
Lian, Wei
Chen, Jieting
Li, Wenlei
Li, Lingyun
Huang, Zhong
author_sort Xu, Jianyong
collection PubMed
description OBJECTIVES: Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet. MATERIALS AND METHODS: Here, we developed the full chemical-defined medium, NBVbe medium, via RNA sequencing, bioinformatic analysis, and growth factor screening. RESULTS: The NBVbe medium contains N2B27 medium with the BSA (bovine serum albumin) replaced by the recombinant human albumin, bFGF (basic fibroblast growth factor), vitamin C, and EGF (epidermal growth factor). The NBVbe medium could support the isolation and expansion of human MSCs from the umbilical cords. CONCLUSIONS: The full chemical-defined medium supporting the isolation and expansion of human MSCs has been developed. This would be helpful for further optimization of the MSC medium, their clinical applications, and molecular characterization.
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spelling pubmed-70830662020-03-23 Chemical-defined medium supporting the expansion of human mesenchymal stem cells Xu, Jianyong Lian, Wei Chen, Jieting Li, Wenlei Li, Lingyun Huang, Zhong Stem Cell Res Ther Research OBJECTIVES: Mesenchymal stem cells (MSCs) have been intensively investigated as to their therapeutic potentials. However, the full chemical-defined medium supporting the isolation and expansion of human MSCs has not been developed yet. MATERIALS AND METHODS: Here, we developed the full chemical-defined medium, NBVbe medium, via RNA sequencing, bioinformatic analysis, and growth factor screening. RESULTS: The NBVbe medium contains N2B27 medium with the BSA (bovine serum albumin) replaced by the recombinant human albumin, bFGF (basic fibroblast growth factor), vitamin C, and EGF (epidermal growth factor). The NBVbe medium could support the isolation and expansion of human MSCs from the umbilical cords. CONCLUSIONS: The full chemical-defined medium supporting the isolation and expansion of human MSCs has been developed. This would be helpful for further optimization of the MSC medium, their clinical applications, and molecular characterization. BioMed Central 2020-03-19 /pmc/articles/PMC7083066/ /pubmed/32192530 http://dx.doi.org/10.1186/s13287-020-01641-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Xu, Jianyong
Lian, Wei
Chen, Jieting
Li, Wenlei
Li, Lingyun
Huang, Zhong
Chemical-defined medium supporting the expansion of human mesenchymal stem cells
title Chemical-defined medium supporting the expansion of human mesenchymal stem cells
title_full Chemical-defined medium supporting the expansion of human mesenchymal stem cells
title_fullStr Chemical-defined medium supporting the expansion of human mesenchymal stem cells
title_full_unstemmed Chemical-defined medium supporting the expansion of human mesenchymal stem cells
title_short Chemical-defined medium supporting the expansion of human mesenchymal stem cells
title_sort chemical-defined medium supporting the expansion of human mesenchymal stem cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083066/
https://www.ncbi.nlm.nih.gov/pubmed/32192530
http://dx.doi.org/10.1186/s13287-020-01641-7
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