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Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials
Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-p...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083606/ https://www.ncbi.nlm.nih.gov/pubmed/32219161 http://dx.doi.org/10.1126/sciadv.aay4361 |
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author | Hussey, George S. Pineda Molina, Catalina Cramer, Madeline C. Tyurina, Yulia Y. Tyurin, Vladimir A. Lee, Yoojin C. El-Mossier, Salma O. Murdock, Mark H. Timashev, Peter S. Kagan, Valerian E. Badylak, Stephen F. |
author_facet | Hussey, George S. Pineda Molina, Catalina Cramer, Madeline C. Tyurina, Yulia Y. Tyurin, Vladimir A. Lee, Yoojin C. El-Mossier, Salma O. Murdock, Mark H. Timashev, Peter S. Kagan, Valerian E. Badylak, Stephen F. |
author_sort | Hussey, George S. |
collection | PubMed |
description | Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography–mass spectrometry (LC-MS)–based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM. |
format | Online Article Text |
id | pubmed-7083606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-70836062020-03-26 Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials Hussey, George S. Pineda Molina, Catalina Cramer, Madeline C. Tyurina, Yulia Y. Tyurin, Vladimir A. Lee, Yoojin C. El-Mossier, Salma O. Murdock, Mark H. Timashev, Peter S. Kagan, Valerian E. Badylak, Stephen F. Sci Adv Research Articles Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography–mass spectrometry (LC-MS)–based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM. American Association for the Advancement of Science 2020-03-20 /pmc/articles/PMC7083606/ /pubmed/32219161 http://dx.doi.org/10.1126/sciadv.aay4361 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Hussey, George S. Pineda Molina, Catalina Cramer, Madeline C. Tyurina, Yulia Y. Tyurin, Vladimir A. Lee, Yoojin C. El-Mossier, Salma O. Murdock, Mark H. Timashev, Peter S. Kagan, Valerian E. Badylak, Stephen F. Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials |
title | Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials |
title_full | Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials |
title_fullStr | Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials |
title_full_unstemmed | Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials |
title_short | Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials |
title_sort | lipidomics and rna sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083606/ https://www.ncbi.nlm.nih.gov/pubmed/32219161 http://dx.doi.org/10.1126/sciadv.aay4361 |
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