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Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach
The immense application potential of amphiphilic protein-polymer conjugates remains largely unexplored, as established “grafting from” synthetic protocols involve time-consuming, harsh and disruptive deoxygenation methods, while “grafting to” approaches result in low yields. Here we report an oxygen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083936/ https://www.ncbi.nlm.nih.gov/pubmed/32198365 http://dx.doi.org/10.1038/s41467-020-15259-z |
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author | Theodorou, Alexis Liarou, Evelina Haddleton, David M. Stavrakaki, Iren Georgia Skordalidis, Panagiotis Whitfield, Richard Anastasaki, Athina Velonia, Kelly |
author_facet | Theodorou, Alexis Liarou, Evelina Haddleton, David M. Stavrakaki, Iren Georgia Skordalidis, Panagiotis Whitfield, Richard Anastasaki, Athina Velonia, Kelly |
author_sort | Theodorou, Alexis |
collection | PubMed |
description | The immense application potential of amphiphilic protein-polymer conjugates remains largely unexplored, as established “grafting from” synthetic protocols involve time-consuming, harsh and disruptive deoxygenation methods, while “grafting to” approaches result in low yields. Here we report an oxygen tolerant, photoinduced CRP approach which readily affords quantitative yields of protein-polymer conjugates within 2 h, avoiding damage to the secondary structure of the protein and providing easily accessible means to produce biomacromolecular assemblies. Importantly, our methodology is compatible with multiple proteins (e.g. BSA, HSA, GOx, beta-galactosidase) and monomer classes including acrylates, methacrylates, styrenics and acrylamides. The polymerizations are conveniently conducted in plastic syringes and in the absence of any additives or external deoxygenation procedures using low-organic content media and ppm levels of copper. The robustness of the protocol is further exemplified by its implementation under UV, blue light or even sunlight irradiation as well as in buffer, nanopure, tap or even sea water. |
format | Online Article Text |
id | pubmed-7083936 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70839362020-03-23 Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach Theodorou, Alexis Liarou, Evelina Haddleton, David M. Stavrakaki, Iren Georgia Skordalidis, Panagiotis Whitfield, Richard Anastasaki, Athina Velonia, Kelly Nat Commun Article The immense application potential of amphiphilic protein-polymer conjugates remains largely unexplored, as established “grafting from” synthetic protocols involve time-consuming, harsh and disruptive deoxygenation methods, while “grafting to” approaches result in low yields. Here we report an oxygen tolerant, photoinduced CRP approach which readily affords quantitative yields of protein-polymer conjugates within 2 h, avoiding damage to the secondary structure of the protein and providing easily accessible means to produce biomacromolecular assemblies. Importantly, our methodology is compatible with multiple proteins (e.g. BSA, HSA, GOx, beta-galactosidase) and monomer classes including acrylates, methacrylates, styrenics and acrylamides. The polymerizations are conveniently conducted in plastic syringes and in the absence of any additives or external deoxygenation procedures using low-organic content media and ppm levels of copper. The robustness of the protocol is further exemplified by its implementation under UV, blue light or even sunlight irradiation as well as in buffer, nanopure, tap or even sea water. Nature Publishing Group UK 2020-03-20 /pmc/articles/PMC7083936/ /pubmed/32198365 http://dx.doi.org/10.1038/s41467-020-15259-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Theodorou, Alexis Liarou, Evelina Haddleton, David M. Stavrakaki, Iren Georgia Skordalidis, Panagiotis Whitfield, Richard Anastasaki, Athina Velonia, Kelly Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach |
title | Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach |
title_full | Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach |
title_fullStr | Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach |
title_full_unstemmed | Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach |
title_short | Protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach |
title_sort | protein-polymer bioconjugates via a versatile oxygen tolerant photoinduced controlled radical polymerization approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7083936/ https://www.ncbi.nlm.nih.gov/pubmed/32198365 http://dx.doi.org/10.1038/s41467-020-15259-z |
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