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miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway

MicroRNAs are evolutionarily conserved, small non-coding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. We previously found that miR-9-5p is abundantly expressed in chicken skeletal muscle. Here, we demonstrate a new role for miR-9-5p as a myogenic micr...

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Autores principales: Yin, Huadong, He, Haorong, Shen, Xiaoxu, Zhao, Jing, Cao, Xinao, Han, Shunshun, Cui, Can, Chen, Yuqi, Wei, Yuanhang, Xia, Lu, Wang, Yan, Li, Diyan, Zhu, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084337/
https://www.ncbi.nlm.nih.gov/pubmed/32121275
http://dx.doi.org/10.3390/ijms21051655
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author Yin, Huadong
He, Haorong
Shen, Xiaoxu
Zhao, Jing
Cao, Xinao
Han, Shunshun
Cui, Can
Chen, Yuqi
Wei, Yuanhang
Xia, Lu
Wang, Yan
Li, Diyan
Zhu, Qing
author_facet Yin, Huadong
He, Haorong
Shen, Xiaoxu
Zhao, Jing
Cao, Xinao
Han, Shunshun
Cui, Can
Chen, Yuqi
Wei, Yuanhang
Xia, Lu
Wang, Yan
Li, Diyan
Zhu, Qing
author_sort Yin, Huadong
collection PubMed
description MicroRNAs are evolutionarily conserved, small non-coding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. We previously found that miR-9-5p is abundantly expressed in chicken skeletal muscle. Here, we demonstrate a new role for miR-9-5p as a myogenic microRNA that regulates skeletal muscle development. The overexpression of miR-9-5p significantly inhibited the proliferation and differentiation of skeletal muscle satellite cells (SMSCs), whereas miR-9-5p inhibition had the opposite effect. We show that insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) is a target gene of miR-9-5p, using dual-luciferase assays, RT-qPCR, and Western Blotting, and that it promotes proliferation and differentiation of SMSCs. In addition, we found that IGF2BP3 regulates IGF-2 expression, using overexpression and knockdown studies. We show that Akt is activated by IGF2BP3 and is essential for IGF2BP3-induced cell development. Together, our results indicate that miR-9-5p could regulate the proliferation and differentiation of myoblasts by targeting IGF2BP3 through IGF-2 and that this activity results in the activation of the PI3K/Akt signaling pathway in skeletal muscle cells.
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spelling pubmed-70843372020-03-24 miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway Yin, Huadong He, Haorong Shen, Xiaoxu Zhao, Jing Cao, Xinao Han, Shunshun Cui, Can Chen, Yuqi Wei, Yuanhang Xia, Lu Wang, Yan Li, Diyan Zhu, Qing Int J Mol Sci Article MicroRNAs are evolutionarily conserved, small non-coding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. We previously found that miR-9-5p is abundantly expressed in chicken skeletal muscle. Here, we demonstrate a new role for miR-9-5p as a myogenic microRNA that regulates skeletal muscle development. The overexpression of miR-9-5p significantly inhibited the proliferation and differentiation of skeletal muscle satellite cells (SMSCs), whereas miR-9-5p inhibition had the opposite effect. We show that insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) is a target gene of miR-9-5p, using dual-luciferase assays, RT-qPCR, and Western Blotting, and that it promotes proliferation and differentiation of SMSCs. In addition, we found that IGF2BP3 regulates IGF-2 expression, using overexpression and knockdown studies. We show that Akt is activated by IGF2BP3 and is essential for IGF2BP3-induced cell development. Together, our results indicate that miR-9-5p could regulate the proliferation and differentiation of myoblasts by targeting IGF2BP3 through IGF-2 and that this activity results in the activation of the PI3K/Akt signaling pathway in skeletal muscle cells. MDPI 2020-02-28 /pmc/articles/PMC7084337/ /pubmed/32121275 http://dx.doi.org/10.3390/ijms21051655 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yin, Huadong
He, Haorong
Shen, Xiaoxu
Zhao, Jing
Cao, Xinao
Han, Shunshun
Cui, Can
Chen, Yuqi
Wei, Yuanhang
Xia, Lu
Wang, Yan
Li, Diyan
Zhu, Qing
miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway
title miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway
title_full miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway
title_fullStr miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway
title_full_unstemmed miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway
title_short miR-9-5p Inhibits Skeletal Muscle Satellite Cell Proliferation and Differentiation by Targeting IGF2BP3 through the IGF2-PI3K/Akt Signaling Pathway
title_sort mir-9-5p inhibits skeletal muscle satellite cell proliferation and differentiation by targeting igf2bp3 through the igf2-pi3k/akt signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084337/
https://www.ncbi.nlm.nih.gov/pubmed/32121275
http://dx.doi.org/10.3390/ijms21051655
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