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The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models
Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084520/ https://www.ncbi.nlm.nih.gov/pubmed/32164182 http://dx.doi.org/10.3390/ijms21051882 |
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author | Rosignol, Ines Villarejo-Zori, Beatriz Teresak, Petra Sierra-Filardi, Elena Pereiro, Xandra Rodríguez-Muela, Natalia Vecino, Elena Vieira, Helena L. A. Bell, Katharina Boya, Patricia |
author_facet | Rosignol, Ines Villarejo-Zori, Beatriz Teresak, Petra Sierra-Filardi, Elena Pereiro, Xandra Rodríguez-Muela, Natalia Vecino, Elena Vieira, Helena L. A. Bell, Katharina Boya, Patricia |
author_sort | Rosignol, Ines |
collection | PubMed |
description | Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing mitophagy in vivo. We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito-QC mice to quantify mitophagy activation in vitro and ex vivo. We also analyzed mitophagy in retinal ganglion cells (RGCs), in vivo, using different mitophagy inducers, as well as after optic nerve crush (ONC) in mice, a commonly used surgical procedure to model glaucoma. Using mito-QC reporter we quantified mitophagy induced by several known inducers in primary RGCs in vitro, ex vivo and in vivo. We also found that RGCs were rescued from some glaucoma relevant stress factors by incubation with the iron chelator deferiprone (DFP). Thus, the mito-QC reporter-based model is a valuable tool for accurately analyzing mitophagy in the context of glaucoma. |
format | Online Article Text |
id | pubmed-7084520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70845202020-03-24 The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models Rosignol, Ines Villarejo-Zori, Beatriz Teresak, Petra Sierra-Filardi, Elena Pereiro, Xandra Rodríguez-Muela, Natalia Vecino, Elena Vieira, Helena L. A. Bell, Katharina Boya, Patricia Int J Mol Sci Article Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing mitophagy in vivo. We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito-QC mice to quantify mitophagy activation in vitro and ex vivo. We also analyzed mitophagy in retinal ganglion cells (RGCs), in vivo, using different mitophagy inducers, as well as after optic nerve crush (ONC) in mice, a commonly used surgical procedure to model glaucoma. Using mito-QC reporter we quantified mitophagy induced by several known inducers in primary RGCs in vitro, ex vivo and in vivo. We also found that RGCs were rescued from some glaucoma relevant stress factors by incubation with the iron chelator deferiprone (DFP). Thus, the mito-QC reporter-based model is a valuable tool for accurately analyzing mitophagy in the context of glaucoma. MDPI 2020-03-10 /pmc/articles/PMC7084520/ /pubmed/32164182 http://dx.doi.org/10.3390/ijms21051882 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rosignol, Ines Villarejo-Zori, Beatriz Teresak, Petra Sierra-Filardi, Elena Pereiro, Xandra Rodríguez-Muela, Natalia Vecino, Elena Vieira, Helena L. A. Bell, Katharina Boya, Patricia The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models |
title | The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models |
title_full | The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models |
title_fullStr | The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models |
title_full_unstemmed | The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models |
title_short | The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models |
title_sort | mito-qc reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084520/ https://www.ncbi.nlm.nih.gov/pubmed/32164182 http://dx.doi.org/10.3390/ijms21051882 |
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