Cargando…

The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models

Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing...

Descripción completa

Detalles Bibliográficos
Autores principales: Rosignol, Ines, Villarejo-Zori, Beatriz, Teresak, Petra, Sierra-Filardi, Elena, Pereiro, Xandra, Rodríguez-Muela, Natalia, Vecino, Elena, Vieira, Helena L. A., Bell, Katharina, Boya, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084520/
https://www.ncbi.nlm.nih.gov/pubmed/32164182
http://dx.doi.org/10.3390/ijms21051882
_version_ 1783508740860805120
author Rosignol, Ines
Villarejo-Zori, Beatriz
Teresak, Petra
Sierra-Filardi, Elena
Pereiro, Xandra
Rodríguez-Muela, Natalia
Vecino, Elena
Vieira, Helena L. A.
Bell, Katharina
Boya, Patricia
author_facet Rosignol, Ines
Villarejo-Zori, Beatriz
Teresak, Petra
Sierra-Filardi, Elena
Pereiro, Xandra
Rodríguez-Muela, Natalia
Vecino, Elena
Vieira, Helena L. A.
Bell, Katharina
Boya, Patricia
author_sort Rosignol, Ines
collection PubMed
description Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing mitophagy in vivo. We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito-QC mice to quantify mitophagy activation in vitro and ex vivo. We also analyzed mitophagy in retinal ganglion cells (RGCs), in vivo, using different mitophagy inducers, as well as after optic nerve crush (ONC) in mice, a commonly used surgical procedure to model glaucoma. Using mito-QC reporter we quantified mitophagy induced by several known inducers in primary RGCs in vitro, ex vivo and in vivo. We also found that RGCs were rescued from some glaucoma relevant stress factors by incubation with the iron chelator deferiprone (DFP). Thus, the mito-QC reporter-based model is a valuable tool for accurately analyzing mitophagy in the context of glaucoma.
format Online
Article
Text
id pubmed-7084520
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70845202020-03-24 The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models Rosignol, Ines Villarejo-Zori, Beatriz Teresak, Petra Sierra-Filardi, Elena Pereiro, Xandra Rodríguez-Muela, Natalia Vecino, Elena Vieira, Helena L. A. Bell, Katharina Boya, Patricia Int J Mol Sci Article Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing mitophagy in vivo. We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito-QC mice to quantify mitophagy activation in vitro and ex vivo. We also analyzed mitophagy in retinal ganglion cells (RGCs), in vivo, using different mitophagy inducers, as well as after optic nerve crush (ONC) in mice, a commonly used surgical procedure to model glaucoma. Using mito-QC reporter we quantified mitophagy induced by several known inducers in primary RGCs in vitro, ex vivo and in vivo. We also found that RGCs were rescued from some glaucoma relevant stress factors by incubation with the iron chelator deferiprone (DFP). Thus, the mito-QC reporter-based model is a valuable tool for accurately analyzing mitophagy in the context of glaucoma. MDPI 2020-03-10 /pmc/articles/PMC7084520/ /pubmed/32164182 http://dx.doi.org/10.3390/ijms21051882 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosignol, Ines
Villarejo-Zori, Beatriz
Teresak, Petra
Sierra-Filardi, Elena
Pereiro, Xandra
Rodríguez-Muela, Natalia
Vecino, Elena
Vieira, Helena L. A.
Bell, Katharina
Boya, Patricia
The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models
title The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models
title_full The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models
title_fullStr The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models
title_full_unstemmed The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models
title_short The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models
title_sort mito-qc reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084520/
https://www.ncbi.nlm.nih.gov/pubmed/32164182
http://dx.doi.org/10.3390/ijms21051882
work_keys_str_mv AT rosignolines themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT villarejozoribeatriz themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT teresakpetra themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT sierrafilardielena themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT pereiroxandra themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT rodriguezmuelanatalia themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT vecinoelena themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT vieirahelenala themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT bellkatharina themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT boyapatricia themitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT rosignolines mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT villarejozoribeatriz mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT teresakpetra mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT sierrafilardielena mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT pereiroxandra mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT rodriguezmuelanatalia mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT vecinoelena mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT vieirahelenala mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT bellkatharina mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels
AT boyapatricia mitoqcreporterforquantitativemitophagyassessmentinprimaryretinalganglioncellsandexperimentalglaucomamodels