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Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis

Cross-linking of antigen-specific IgE bound to the high-affinity IgE receptor (FcεRI) on the surface of mast cells with multivalent antigens results in the release of mediators and development of type 2 inflammation. FcεRI expression and IgE synthesis are, therefore, critical for type 2 inflammatory...

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Autores principales: Gion, Yuka, Okano, Mitsuhiro, Koyama, Takahisa, Oura, Tokie, Nishikori, Asami, Orita, Yorihisa, Tachibana, Tomoyasu, Marunaka, Hidenori, Makino, Takuma, Nishizaki, Kazunori, Sato, Yasuharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084524/
https://www.ncbi.nlm.nih.gov/pubmed/32155995
http://dx.doi.org/10.3390/ijms21051843
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author Gion, Yuka
Okano, Mitsuhiro
Koyama, Takahisa
Oura, Tokie
Nishikori, Asami
Orita, Yorihisa
Tachibana, Tomoyasu
Marunaka, Hidenori
Makino, Takuma
Nishizaki, Kazunori
Sato, Yasuharu
author_facet Gion, Yuka
Okano, Mitsuhiro
Koyama, Takahisa
Oura, Tokie
Nishikori, Asami
Orita, Yorihisa
Tachibana, Tomoyasu
Marunaka, Hidenori
Makino, Takuma
Nishizaki, Kazunori
Sato, Yasuharu
author_sort Gion, Yuka
collection PubMed
description Cross-linking of antigen-specific IgE bound to the high-affinity IgE receptor (FcεRI) on the surface of mast cells with multivalent antigens results in the release of mediators and development of type 2 inflammation. FcεRI expression and IgE synthesis are, therefore, critical for type 2 inflammatory disease development. In an attempt to clarify the relationship between eosinophilic chronic rhinosinusitis (ECRS) and mast cell infiltration, we analyzed mast cell infiltration at lesion sites and determined its clinical significance. Mast cells are positive for c-kit, and IgE in uncinated tissues (UT) and nasal polyps (NP) were examined by immunohistochemistry. The number of positive cells and clinicopathological factors were analyzed. Patients with ECRS exhibited high levels of total IgE serum levels and elevated peripheral blood eosinophil ratios. As a result, the number of mast cells with membranes positive for c-kit and IgE increased significantly in lesions forming NP. Therefore, we classified IgE-positive mast cells into two groups: membrane IgE-positive cells and cytoplasmic IgE-positive cells. The amount of membrane IgE-positive mast cells was significantly increased in moderate ECRS. A positive correlation was found between the membrane IgE-positive cells and the radiological severity score, the ratio of eosinophils, and the total serum IgE level. The number of cytoplasmic IgE-positive mast cells was significantly increased in moderate and severe ECRS. A positive correlation was observed between the cytoplasmic IgE-positive cells and the radiological severity score, the ratio of eosinophils in the blood, and the total IgE level. These results suggest that the process of mast cell internalization of antigens via the IgE receptor is involved in ECRS pathogenesis.
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spelling pubmed-70845242020-03-24 Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis Gion, Yuka Okano, Mitsuhiro Koyama, Takahisa Oura, Tokie Nishikori, Asami Orita, Yorihisa Tachibana, Tomoyasu Marunaka, Hidenori Makino, Takuma Nishizaki, Kazunori Sato, Yasuharu Int J Mol Sci Communication Cross-linking of antigen-specific IgE bound to the high-affinity IgE receptor (FcεRI) on the surface of mast cells with multivalent antigens results in the release of mediators and development of type 2 inflammation. FcεRI expression and IgE synthesis are, therefore, critical for type 2 inflammatory disease development. In an attempt to clarify the relationship between eosinophilic chronic rhinosinusitis (ECRS) and mast cell infiltration, we analyzed mast cell infiltration at lesion sites and determined its clinical significance. Mast cells are positive for c-kit, and IgE in uncinated tissues (UT) and nasal polyps (NP) were examined by immunohistochemistry. The number of positive cells and clinicopathological factors were analyzed. Patients with ECRS exhibited high levels of total IgE serum levels and elevated peripheral blood eosinophil ratios. As a result, the number of mast cells with membranes positive for c-kit and IgE increased significantly in lesions forming NP. Therefore, we classified IgE-positive mast cells into two groups: membrane IgE-positive cells and cytoplasmic IgE-positive cells. The amount of membrane IgE-positive mast cells was significantly increased in moderate ECRS. A positive correlation was found between the membrane IgE-positive cells and the radiological severity score, the ratio of eosinophils, and the total serum IgE level. The number of cytoplasmic IgE-positive mast cells was significantly increased in moderate and severe ECRS. A positive correlation was observed between the cytoplasmic IgE-positive cells and the radiological severity score, the ratio of eosinophils in the blood, and the total IgE level. These results suggest that the process of mast cell internalization of antigens via the IgE receptor is involved in ECRS pathogenesis. MDPI 2020-03-07 /pmc/articles/PMC7084524/ /pubmed/32155995 http://dx.doi.org/10.3390/ijms21051843 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Gion, Yuka
Okano, Mitsuhiro
Koyama, Takahisa
Oura, Tokie
Nishikori, Asami
Orita, Yorihisa
Tachibana, Tomoyasu
Marunaka, Hidenori
Makino, Takuma
Nishizaki, Kazunori
Sato, Yasuharu
Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis
title Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis
title_full Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis
title_fullStr Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis
title_full_unstemmed Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis
title_short Clinical Significance of Cytoplasmic IgE-Positive Mast Cells in Eosinophilic Chronic Rhinosinusitis
title_sort clinical significance of cytoplasmic ige-positive mast cells in eosinophilic chronic rhinosinusitis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084524/
https://www.ncbi.nlm.nih.gov/pubmed/32155995
http://dx.doi.org/10.3390/ijms21051843
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