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One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging
Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance....
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084562/ https://www.ncbi.nlm.nih.gov/pubmed/32121128 http://dx.doi.org/10.3390/ma13051076 |
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author | Pinto, Ricardo J. B. Bispo, Daniela Vilela, Carla Botas, Alexandre M. P. Ferreira, Rute A. S. Menezes, Ana C. Campos, Fábio Oliveira, Helena Abreu, Maria H. Santos, Sónia A. O. Freire, Carmen S. R. |
author_facet | Pinto, Ricardo J. B. Bispo, Daniela Vilela, Carla Botas, Alexandre M. P. Ferreira, Rute A. S. Menezes, Ana C. Campos, Fábio Oliveira, Helena Abreu, Maria H. Santos, Sónia A. O. Freire, Carmen S. R. |
author_sort | Pinto, Ricardo J. B. |
collection | PubMed |
description | Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance. Thus, the present study reports a one-minute microwave-assisted synthesis of fucoidan-coated AuNPs with controllable size and high antitumoral activity. The NPs were synthesized using a fucoidan-enriched fraction extracted from Fucus vesiculosus, as the reducing and capping agent. The ensuing monodispersed and spherical NPs exhibit tiny diameters between 5.8 and 13.4 nm for concentrations of fucoidan between 0.5 and 0.05% (w/v), respectively, as excellent colloidal stability in distinct solutions and culture media. Furthermore, the NPs present antitumoral activity against three human tumor cell lines (MNT-1, HepG2, and MG-63), and flow cytometry in combination with dark-field imaging confirmed the cellular uptake of NPs by MG-63 cell line. |
format | Online Article Text |
id | pubmed-7084562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70845622020-03-24 One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging Pinto, Ricardo J. B. Bispo, Daniela Vilela, Carla Botas, Alexandre M. P. Ferreira, Rute A. S. Menezes, Ana C. Campos, Fábio Oliveira, Helena Abreu, Maria H. Santos, Sónia A. O. Freire, Carmen S. R. Materials (Basel) Article Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance. Thus, the present study reports a one-minute microwave-assisted synthesis of fucoidan-coated AuNPs with controllable size and high antitumoral activity. The NPs were synthesized using a fucoidan-enriched fraction extracted from Fucus vesiculosus, as the reducing and capping agent. The ensuing monodispersed and spherical NPs exhibit tiny diameters between 5.8 and 13.4 nm for concentrations of fucoidan between 0.5 and 0.05% (w/v), respectively, as excellent colloidal stability in distinct solutions and culture media. Furthermore, the NPs present antitumoral activity against three human tumor cell lines (MNT-1, HepG2, and MG-63), and flow cytometry in combination with dark-field imaging confirmed the cellular uptake of NPs by MG-63 cell line. MDPI 2020-02-28 /pmc/articles/PMC7084562/ /pubmed/32121128 http://dx.doi.org/10.3390/ma13051076 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pinto, Ricardo J. B. Bispo, Daniela Vilela, Carla Botas, Alexandre M. P. Ferreira, Rute A. S. Menezes, Ana C. Campos, Fábio Oliveira, Helena Abreu, Maria H. Santos, Sónia A. O. Freire, Carmen S. R. One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging |
title | One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging |
title_full | One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging |
title_fullStr | One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging |
title_full_unstemmed | One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging |
title_short | One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging |
title_sort | one-minute synthesis of size-controlled fucoidan-gold nanosystems: antitumoral activity and dark field imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084562/ https://www.ncbi.nlm.nih.gov/pubmed/32121128 http://dx.doi.org/10.3390/ma13051076 |
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