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A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma

This paper aims to validate if intrapancreatic injection of penicillin G can enhance hardness and suture holding capacity (SHC) of the pancreas through prompting the fibrosis process. Soft pancreatic texture is constantly mentioned as one of the most contributory predictors of postoperative pancreat...

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Autores principales: Lee, Sang Chul, Hong, Tae Ho, Kim, Ok-Hee, Cho, Suk Joon, Kim, Kee-Hwan, Song, Jin Sook, Hwang, Kyu-Seok, Jung, Jae-Kyung, Hong, Ha-Eun, Seo, Haeyeon, Choi, Ho Joong, Ahn, Joseph, Lee, Tae Yoon, Rim, Eunyoung, Jung, Kwan-Young, Kim, Say-June
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084673/
https://www.ncbi.nlm.nih.gov/pubmed/32143463
http://dx.doi.org/10.3390/ijms21051759
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author Lee, Sang Chul
Hong, Tae Ho
Kim, Ok-Hee
Cho, Suk Joon
Kim, Kee-Hwan
Song, Jin Sook
Hwang, Kyu-Seok
Jung, Jae-Kyung
Hong, Ha-Eun
Seo, Haeyeon
Choi, Ho Joong
Ahn, Joseph
Lee, Tae Yoon
Rim, Eunyoung
Jung, Kwan-Young
Kim, Say-June
author_facet Lee, Sang Chul
Hong, Tae Ho
Kim, Ok-Hee
Cho, Suk Joon
Kim, Kee-Hwan
Song, Jin Sook
Hwang, Kyu-Seok
Jung, Jae-Kyung
Hong, Ha-Eun
Seo, Haeyeon
Choi, Ho Joong
Ahn, Joseph
Lee, Tae Yoon
Rim, Eunyoung
Jung, Kwan-Young
Kim, Say-June
author_sort Lee, Sang Chul
collection PubMed
description This paper aims to validate if intrapancreatic injection of penicillin G can enhance hardness and suture holding capacity (SHC) of the pancreas through prompting the fibrosis process. Soft pancreatic texture is constantly mentioned as one of the most contributory predictors of postoperative pancreatic fistula (POPF). Soft pancreas has poor SHC and higher incidence of parenchymal tearing, frequently leading to POPF. From a library of 114 antibiotic compounds, we identified that penicillin G substantially enhanced pancreatic hardness and SHC in experimental mice. Specifically, we injected penicillin G directly into the pancreas. On determined dates, we measured the pancreatic hardness and SHC, respectively, and performed molecular and histological examinations for estimation of the degree of fibrosis. The intrapancreatic injection of penicillin G activated human pancreatic stellate cells (HPSCs) to produce various fibrotic materials such as transforming growth factor-β1 (TGF-β1) and metalloproteinases-2. The pancreatic hardness and SHC were increased to the maximum at the second day after injection and then it gradually subsided demonstrating its reversibility. Pretreatment of mice with SB431542, an inhibitor of the TGF-β1 receptor, before injecting penicillin G intrapancreatically, significantly abrogated the increase of both pancreatic hardness and SHC caused by penicillin G. This suggested that penicillin G promotes pancreatic fibrosis through the TGF-β1 signaling pathway. Intrapancreatic injection of penicillin G promotes pancreatic hardness and SHC by enhancing pancreatic fibrosis. We thus think that penicillin G could be utilized to prevent and minimize POPF, after validating its actual effectiveness and safety by further studies.
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spelling pubmed-70846732020-03-24 A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma Lee, Sang Chul Hong, Tae Ho Kim, Ok-Hee Cho, Suk Joon Kim, Kee-Hwan Song, Jin Sook Hwang, Kyu-Seok Jung, Jae-Kyung Hong, Ha-Eun Seo, Haeyeon Choi, Ho Joong Ahn, Joseph Lee, Tae Yoon Rim, Eunyoung Jung, Kwan-Young Kim, Say-June Int J Mol Sci Article This paper aims to validate if intrapancreatic injection of penicillin G can enhance hardness and suture holding capacity (SHC) of the pancreas through prompting the fibrosis process. Soft pancreatic texture is constantly mentioned as one of the most contributory predictors of postoperative pancreatic fistula (POPF). Soft pancreas has poor SHC and higher incidence of parenchymal tearing, frequently leading to POPF. From a library of 114 antibiotic compounds, we identified that penicillin G substantially enhanced pancreatic hardness and SHC in experimental mice. Specifically, we injected penicillin G directly into the pancreas. On determined dates, we measured the pancreatic hardness and SHC, respectively, and performed molecular and histological examinations for estimation of the degree of fibrosis. The intrapancreatic injection of penicillin G activated human pancreatic stellate cells (HPSCs) to produce various fibrotic materials such as transforming growth factor-β1 (TGF-β1) and metalloproteinases-2. The pancreatic hardness and SHC were increased to the maximum at the second day after injection and then it gradually subsided demonstrating its reversibility. Pretreatment of mice with SB431542, an inhibitor of the TGF-β1 receptor, before injecting penicillin G intrapancreatically, significantly abrogated the increase of both pancreatic hardness and SHC caused by penicillin G. This suggested that penicillin G promotes pancreatic fibrosis through the TGF-β1 signaling pathway. Intrapancreatic injection of penicillin G promotes pancreatic hardness and SHC by enhancing pancreatic fibrosis. We thus think that penicillin G could be utilized to prevent and minimize POPF, after validating its actual effectiveness and safety by further studies. MDPI 2020-03-04 /pmc/articles/PMC7084673/ /pubmed/32143463 http://dx.doi.org/10.3390/ijms21051759 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Sang Chul
Hong, Tae Ho
Kim, Ok-Hee
Cho, Suk Joon
Kim, Kee-Hwan
Song, Jin Sook
Hwang, Kyu-Seok
Jung, Jae-Kyung
Hong, Ha-Eun
Seo, Haeyeon
Choi, Ho Joong
Ahn, Joseph
Lee, Tae Yoon
Rim, Eunyoung
Jung, Kwan-Young
Kim, Say-June
A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma
title A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma
title_full A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma
title_fullStr A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma
title_full_unstemmed A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma
title_short A Novel Way of Preventing Postoperative Pancreatic Fistula by Directly Injecting Profibrogenic Materials into the Pancreatic Parenchyma
title_sort novel way of preventing postoperative pancreatic fistula by directly injecting profibrogenic materials into the pancreatic parenchyma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084673/
https://www.ncbi.nlm.nih.gov/pubmed/32143463
http://dx.doi.org/10.3390/ijms21051759
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