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Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency

Ceylon leadwort (Plumbago zeylanica) is ornamental plant known for its pharmacological properties arising from the abundant production of various secondary metabolites. It often grows in lead polluted areas. The aim of presented study was to evaluate the survival strategy of P. zeylanica to lead tox...

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Autores principales: Tokarz, Krzysztof M., Makowski, Wojciech, Tokarz, Barbara, Hanula, Monika, Sitek, Ewa, Muszyńska, Ewa, Jędrzejczyk, Roman, Banasiuk, Rafał, Chajec, Łukasz, Mazur, Stanisław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084747/
https://www.ncbi.nlm.nih.gov/pubmed/32182862
http://dx.doi.org/10.3390/ijms21051866
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author Tokarz, Krzysztof M.
Makowski, Wojciech
Tokarz, Barbara
Hanula, Monika
Sitek, Ewa
Muszyńska, Ewa
Jędrzejczyk, Roman
Banasiuk, Rafał
Chajec, Łukasz
Mazur, Stanisław
author_facet Tokarz, Krzysztof M.
Makowski, Wojciech
Tokarz, Barbara
Hanula, Monika
Sitek, Ewa
Muszyńska, Ewa
Jędrzejczyk, Roman
Banasiuk, Rafał
Chajec, Łukasz
Mazur, Stanisław
author_sort Tokarz, Krzysztof M.
collection PubMed
description Ceylon leadwort (Plumbago zeylanica) is ornamental plant known for its pharmacological properties arising from the abundant production of various secondary metabolites. It often grows in lead polluted areas. The aim of presented study was to evaluate the survival strategy of P. zeylanica to lead toxicity via photosynthetic apparatus acclimatization. Shoots of P. zeylanica were cultivated on media with different Pb concentrations (0.0, 0.05, and 0.1 g Pb∙l(−1)). After a four-week culture, the efficiency of the photosynthetic apparatus of plants was evaluated by Chl a fluorescence measurement, photosynthetic pigment, and Lhcb1, PsbA, PsbO, and RuBisCo protein accumulation, antioxidant enzymes activity, and chloroplast ultrastructure observation. Plants from lower Pb concentration revealed no changes in photosynthetic pigments content and light-harvesting complex (LHCII) size, as well as no limitation on the donor side of Photosystem II Reaction Centre (PSII RC). However, the activity and content of antioxidant enzymes indicated a high risk of limitation on the acceptor side of Photosystem I. In turn, plants from 0.1 g Pb∙l(−1) showed a significant decrease in pigments content, LHCII size, the amount of active PSII RC, oxygen-evolving complex activity, and significant remodeling of chloroplast ultrastructure indicated limitation of PSII RC donor side. Obtained results indicate that P. zeylanica plants acclimate to lead toxicity by Pb accumulation in roots and, depending on Pb concentration, by adjusting their photosynthetic apparatus via the activation of alternative (cyclic and pseudocyclic) electron transport pathways.
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spelling pubmed-70847472020-03-24 Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency Tokarz, Krzysztof M. Makowski, Wojciech Tokarz, Barbara Hanula, Monika Sitek, Ewa Muszyńska, Ewa Jędrzejczyk, Roman Banasiuk, Rafał Chajec, Łukasz Mazur, Stanisław Int J Mol Sci Article Ceylon leadwort (Plumbago zeylanica) is ornamental plant known for its pharmacological properties arising from the abundant production of various secondary metabolites. It often grows in lead polluted areas. The aim of presented study was to evaluate the survival strategy of P. zeylanica to lead toxicity via photosynthetic apparatus acclimatization. Shoots of P. zeylanica were cultivated on media with different Pb concentrations (0.0, 0.05, and 0.1 g Pb∙l(−1)). After a four-week culture, the efficiency of the photosynthetic apparatus of plants was evaluated by Chl a fluorescence measurement, photosynthetic pigment, and Lhcb1, PsbA, PsbO, and RuBisCo protein accumulation, antioxidant enzymes activity, and chloroplast ultrastructure observation. Plants from lower Pb concentration revealed no changes in photosynthetic pigments content and light-harvesting complex (LHCII) size, as well as no limitation on the donor side of Photosystem II Reaction Centre (PSII RC). However, the activity and content of antioxidant enzymes indicated a high risk of limitation on the acceptor side of Photosystem I. In turn, plants from 0.1 g Pb∙l(−1) showed a significant decrease in pigments content, LHCII size, the amount of active PSII RC, oxygen-evolving complex activity, and significant remodeling of chloroplast ultrastructure indicated limitation of PSII RC donor side. Obtained results indicate that P. zeylanica plants acclimate to lead toxicity by Pb accumulation in roots and, depending on Pb concentration, by adjusting their photosynthetic apparatus via the activation of alternative (cyclic and pseudocyclic) electron transport pathways. MDPI 2020-03-09 /pmc/articles/PMC7084747/ /pubmed/32182862 http://dx.doi.org/10.3390/ijms21051866 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tokarz, Krzysztof M.
Makowski, Wojciech
Tokarz, Barbara
Hanula, Monika
Sitek, Ewa
Muszyńska, Ewa
Jędrzejczyk, Roman
Banasiuk, Rafał
Chajec, Łukasz
Mazur, Stanisław
Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency
title Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency
title_full Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency
title_fullStr Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency
title_full_unstemmed Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency
title_short Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?—Studies of Photosynthetic Apparatus Efficiency
title_sort can ceylon leadwort (plumbago zeylanica l.) acclimate to lead toxicity?—studies of photosynthetic apparatus efficiency
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084747/
https://www.ncbi.nlm.nih.gov/pubmed/32182862
http://dx.doi.org/10.3390/ijms21051866
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