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Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes
Mitochondrial respiratory chain supercomplexes (RCS), particularly, the respirasome, which contains complexes I, III, and IV, have been suggested to participate in facilitating electron transport, reducing the production of reactive oxygen species (ROS), and maintaining the structural integrity of i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084778/ https://www.ncbi.nlm.nih.gov/pubmed/32106430 http://dx.doi.org/10.3390/ijms21051555 |
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author | Chapa-Dubocq, Xavier R. Rodríguez-Graciani, Keishla M. Guzmán-Hernández, Roberto A. Jang, Sehwan Brookes, Paul S. Javadov, Sabzali |
author_facet | Chapa-Dubocq, Xavier R. Rodríguez-Graciani, Keishla M. Guzmán-Hernández, Roberto A. Jang, Sehwan Brookes, Paul S. Javadov, Sabzali |
author_sort | Chapa-Dubocq, Xavier R. |
collection | PubMed |
description | Mitochondrial respiratory chain supercomplexes (RCS), particularly, the respirasome, which contains complexes I, III, and IV, have been suggested to participate in facilitating electron transport, reducing the production of reactive oxygen species (ROS), and maintaining the structural integrity of individual electron transport chain (ETC) complexes. Disassembly of the RCS has been observed in Barth syndrome, neurodegenerative and cardiovascular diseases, diabetes mellitus, and aging. However, the physiological role of RCS in high energy-demanding tissues such as the heart remains unknown. This study elucidates the relationship between RCS assembly and cardiac function. Adult male Sprague Dawley rats underwent Langendorff retrograde perfusion in the presence and absence of ethanol, isopropanol, or rotenone (an ETC complex I inhibitor). We found that ethanol had no effects on cardiac function, whereas rotenone reduced heart contractility, which was not recovered when rotenone was excluded from the perfusion medium. Blue native polyacrylamide gel electrophoresis revealed significant reductions of respirasome levels in ethanol- or rotenone-treated groups compared to the control group. In addition, rotenone significantly increased while ethanol had no effect on mitochondrial ROS production. In isolated intact mitochondria in vitro, ethanol did not affect respirasome assembly; however, acetaldehyde, a byproduct of ethanol metabolism, induced dissociation of respirasome. Isopropanol, a secondary alcohol which was used as an alternative compound, had effects similar to ethanol on heart function, respirasome levels, and ROS production. In conclusion, ethanol and isopropanol reduced respirasome levels without any noticeable effect on cardiac parameters, and cardiac function is not susceptible to moderate reductions of RCS. |
format | Online Article Text |
id | pubmed-7084778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70847782020-03-24 Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes Chapa-Dubocq, Xavier R. Rodríguez-Graciani, Keishla M. Guzmán-Hernández, Roberto A. Jang, Sehwan Brookes, Paul S. Javadov, Sabzali Int J Mol Sci Article Mitochondrial respiratory chain supercomplexes (RCS), particularly, the respirasome, which contains complexes I, III, and IV, have been suggested to participate in facilitating electron transport, reducing the production of reactive oxygen species (ROS), and maintaining the structural integrity of individual electron transport chain (ETC) complexes. Disassembly of the RCS has been observed in Barth syndrome, neurodegenerative and cardiovascular diseases, diabetes mellitus, and aging. However, the physiological role of RCS in high energy-demanding tissues such as the heart remains unknown. This study elucidates the relationship between RCS assembly and cardiac function. Adult male Sprague Dawley rats underwent Langendorff retrograde perfusion in the presence and absence of ethanol, isopropanol, or rotenone (an ETC complex I inhibitor). We found that ethanol had no effects on cardiac function, whereas rotenone reduced heart contractility, which was not recovered when rotenone was excluded from the perfusion medium. Blue native polyacrylamide gel electrophoresis revealed significant reductions of respirasome levels in ethanol- or rotenone-treated groups compared to the control group. In addition, rotenone significantly increased while ethanol had no effect on mitochondrial ROS production. In isolated intact mitochondria in vitro, ethanol did not affect respirasome assembly; however, acetaldehyde, a byproduct of ethanol metabolism, induced dissociation of respirasome. Isopropanol, a secondary alcohol which was used as an alternative compound, had effects similar to ethanol on heart function, respirasome levels, and ROS production. In conclusion, ethanol and isopropanol reduced respirasome levels without any noticeable effect on cardiac parameters, and cardiac function is not susceptible to moderate reductions of RCS. MDPI 2020-02-25 /pmc/articles/PMC7084778/ /pubmed/32106430 http://dx.doi.org/10.3390/ijms21051555 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chapa-Dubocq, Xavier R. Rodríguez-Graciani, Keishla M. Guzmán-Hernández, Roberto A. Jang, Sehwan Brookes, Paul S. Javadov, Sabzali Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes |
title | Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes |
title_full | Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes |
title_fullStr | Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes |
title_full_unstemmed | Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes |
title_short | Cardiac Function is not Susceptible to Moderate Disassembly of Mitochondrial Respiratory Supercomplexes |
title_sort | cardiac function is not susceptible to moderate disassembly of mitochondrial respiratory supercomplexes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084778/ https://www.ncbi.nlm.nih.gov/pubmed/32106430 http://dx.doi.org/10.3390/ijms21051555 |
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