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The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins

In the present work, we investigated the response to Cd in Leptodictyum riparium, a cosmopolitan moss (Bryophyta) that can accumulate higher amounts of metals than other plants, even angiosperms, with absence or slight apparent damage. High-performance liquid chromatography followed by electrospray...

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Autores principales: Bellini, Erika, Maresca, Viviana, Betti, Camilla, Castiglione, Monica Ruffini, Fontanini, Debora, Capocchi, Antonella, Sorce, Carlo, Borsò, Marco, Bruno, Laura, Sorbo, Sergio, Basile, Adriana, Sanità di Toppi, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084805/
https://www.ncbi.nlm.nih.gov/pubmed/32111035
http://dx.doi.org/10.3390/ijms21051583
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author Bellini, Erika
Maresca, Viviana
Betti, Camilla
Castiglione, Monica Ruffini
Fontanini, Debora
Capocchi, Antonella
Sorce, Carlo
Borsò, Marco
Bruno, Laura
Sorbo, Sergio
Basile, Adriana
Sanità di Toppi, Luigi
author_facet Bellini, Erika
Maresca, Viviana
Betti, Camilla
Castiglione, Monica Ruffini
Fontanini, Debora
Capocchi, Antonella
Sorce, Carlo
Borsò, Marco
Bruno, Laura
Sorbo, Sergio
Basile, Adriana
Sanità di Toppi, Luigi
author_sort Bellini, Erika
collection PubMed
description In the present work, we investigated the response to Cd in Leptodictyum riparium, a cosmopolitan moss (Bryophyta) that can accumulate higher amounts of metals than other plants, even angiosperms, with absence or slight apparent damage. High-performance liquid chromatography followed by electrospray ionization tandem mass spectrometry of extracts from L. riparium gametophytes, exposed to 0, 36 and 360 µM Cd for 7 days, revealed the presence of γ-glutamylcysteine (γ-EC), reduced glutathione (GSH), and traces of phytochelatins. The increase in Cd concentrations progressively augmented reactive oxygen species levels, with activation of both antioxidant (catalase and superoxide dismutase) and detoxifying (glutathione-S-transferase) enzymes. After Cd treatment, cytosolic and vacuolar localization of thiol peptides was performed by means of the fluorescent dye monochlorobimane and subsequent observation with confocal laser scanning microscopy. The cytosolic fluorescence observed with the highest Cd concentrations was also consistent with the formation of γ-EC-bimane in the cytosol, possibly catalyzed by the peptidase activity of the L. riparium phytochelatin synthase. On the whole, activation of phytochelatin synthase and glutathione-S-transferase, but minimally phytochelatin synthesis, play a role to counteract Cd toxicity in L. riparium, in this manner minimizing the cellular damage caused by the metal. This study strengthens previous investigations on the L. riparium ability to efficiently hinder metal pollution, hinting at a potential use for biomonitoring and phytoremediation purposes.
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spelling pubmed-70848052020-03-24 The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins Bellini, Erika Maresca, Viviana Betti, Camilla Castiglione, Monica Ruffini Fontanini, Debora Capocchi, Antonella Sorce, Carlo Borsò, Marco Bruno, Laura Sorbo, Sergio Basile, Adriana Sanità di Toppi, Luigi Int J Mol Sci Article In the present work, we investigated the response to Cd in Leptodictyum riparium, a cosmopolitan moss (Bryophyta) that can accumulate higher amounts of metals than other plants, even angiosperms, with absence or slight apparent damage. High-performance liquid chromatography followed by electrospray ionization tandem mass spectrometry of extracts from L. riparium gametophytes, exposed to 0, 36 and 360 µM Cd for 7 days, revealed the presence of γ-glutamylcysteine (γ-EC), reduced glutathione (GSH), and traces of phytochelatins. The increase in Cd concentrations progressively augmented reactive oxygen species levels, with activation of both antioxidant (catalase and superoxide dismutase) and detoxifying (glutathione-S-transferase) enzymes. After Cd treatment, cytosolic and vacuolar localization of thiol peptides was performed by means of the fluorescent dye monochlorobimane and subsequent observation with confocal laser scanning microscopy. The cytosolic fluorescence observed with the highest Cd concentrations was also consistent with the formation of γ-EC-bimane in the cytosol, possibly catalyzed by the peptidase activity of the L. riparium phytochelatin synthase. On the whole, activation of phytochelatin synthase and glutathione-S-transferase, but minimally phytochelatin synthesis, play a role to counteract Cd toxicity in L. riparium, in this manner minimizing the cellular damage caused by the metal. This study strengthens previous investigations on the L. riparium ability to efficiently hinder metal pollution, hinting at a potential use for biomonitoring and phytoremediation purposes. MDPI 2020-02-26 /pmc/articles/PMC7084805/ /pubmed/32111035 http://dx.doi.org/10.3390/ijms21051583 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bellini, Erika
Maresca, Viviana
Betti, Camilla
Castiglione, Monica Ruffini
Fontanini, Debora
Capocchi, Antonella
Sorce, Carlo
Borsò, Marco
Bruno, Laura
Sorbo, Sergio
Basile, Adriana
Sanità di Toppi, Luigi
The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins
title The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins
title_full The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins
title_fullStr The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins
title_full_unstemmed The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins
title_short The Moss Leptodictyum riparium Counteracts Severe Cadmium Stress by Activation of Glutathione Transferase and Phytochelatin Synthase, but Slightly by Phytochelatins
title_sort moss leptodictyum riparium counteracts severe cadmium stress by activation of glutathione transferase and phytochelatin synthase, but slightly by phytochelatins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7084805/
https://www.ncbi.nlm.nih.gov/pubmed/32111035
http://dx.doi.org/10.3390/ijms21051583
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