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Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture

Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ(1-42) in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10(-7) M) in astrocytes in primary culture in presence o...

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Detalles Bibliográficos
Autores principales: Jorda, Adrian, Aldasoro, Martin, Aldasoro, Constanza, Guerra-Ojeda, Sol, Iradi, Antonio, Vila, Jose Mª, Campos-Campos, Juan, Valles, Soraya L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085272/
https://www.ncbi.nlm.nih.gov/pubmed/32218705
http://dx.doi.org/10.7150/ijms.40959
Descripción
Sumario:Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ(1-42) in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10(-7) M) in astrocytes in primary culture in presence or absence of Aβ(1-42) toxic peptide. We noted an increase of cell viability and proliferation with or without Aβ(1-42) peptide presence in aspirin treated cells. In addition, a decrease in apoptosis, determined by Caspase 3 activity and the expression of Cyt c and Smac/Diablo, were detected. Also, aspirin diminished necrosis process (LDH levels), pro-inflammatory mediators (IL-β and TNF-α) and NF-ᴋB protein expression, increasing anti-inflammatory PPAR-γ protein expression, preventing Aβ(1-42) toxic effects. Aspirin inhibited COX-2 and iNOS without changes in COX-1 expression, increasing anti-oxidant protein (Cu/Zn-SOD and Mn-SOD) expression in presence or absence of Aβ(1-42). Taken together, our results show that aspirin, at low doses increases cell viability by decreasing inflammation and oxidative stress, preventing the deleterious effects of the Aβ(1-42) peptide on astrocytes in primary culture. The use of low doses of aspirin may be more suitable for Alzheimer's disease.