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Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture
Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ(1-42) in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10(-7) M) in astrocytes in primary culture in presence o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085272/ https://www.ncbi.nlm.nih.gov/pubmed/32218705 http://dx.doi.org/10.7150/ijms.40959 |
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author | Jorda, Adrian Aldasoro, Martin Aldasoro, Constanza Guerra-Ojeda, Sol Iradi, Antonio Vila, Jose Mª Campos-Campos, Juan Valles, Soraya L. |
author_facet | Jorda, Adrian Aldasoro, Martin Aldasoro, Constanza Guerra-Ojeda, Sol Iradi, Antonio Vila, Jose Mª Campos-Campos, Juan Valles, Soraya L. |
author_sort | Jorda, Adrian |
collection | PubMed |
description | Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ(1-42) in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10(-7) M) in astrocytes in primary culture in presence or absence of Aβ(1-42) toxic peptide. We noted an increase of cell viability and proliferation with or without Aβ(1-42) peptide presence in aspirin treated cells. In addition, a decrease in apoptosis, determined by Caspase 3 activity and the expression of Cyt c and Smac/Diablo, were detected. Also, aspirin diminished necrosis process (LDH levels), pro-inflammatory mediators (IL-β and TNF-α) and NF-ᴋB protein expression, increasing anti-inflammatory PPAR-γ protein expression, preventing Aβ(1-42) toxic effects. Aspirin inhibited COX-2 and iNOS without changes in COX-1 expression, increasing anti-oxidant protein (Cu/Zn-SOD and Mn-SOD) expression in presence or absence of Aβ(1-42). Taken together, our results show that aspirin, at low doses increases cell viability by decreasing inflammation and oxidative stress, preventing the deleterious effects of the Aβ(1-42) peptide on astrocytes in primary culture. The use of low doses of aspirin may be more suitable for Alzheimer's disease. |
format | Online Article Text |
id | pubmed-7085272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-70852722020-03-26 Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture Jorda, Adrian Aldasoro, Martin Aldasoro, Constanza Guerra-Ojeda, Sol Iradi, Antonio Vila, Jose Mª Campos-Campos, Juan Valles, Soraya L. Int J Med Sci Research Paper Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ(1-42) in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10(-7) M) in astrocytes in primary culture in presence or absence of Aβ(1-42) toxic peptide. We noted an increase of cell viability and proliferation with or without Aβ(1-42) peptide presence in aspirin treated cells. In addition, a decrease in apoptosis, determined by Caspase 3 activity and the expression of Cyt c and Smac/Diablo, were detected. Also, aspirin diminished necrosis process (LDH levels), pro-inflammatory mediators (IL-β and TNF-α) and NF-ᴋB protein expression, increasing anti-inflammatory PPAR-γ protein expression, preventing Aβ(1-42) toxic effects. Aspirin inhibited COX-2 and iNOS without changes in COX-1 expression, increasing anti-oxidant protein (Cu/Zn-SOD and Mn-SOD) expression in presence or absence of Aβ(1-42). Taken together, our results show that aspirin, at low doses increases cell viability by decreasing inflammation and oxidative stress, preventing the deleterious effects of the Aβ(1-42) peptide on astrocytes in primary culture. The use of low doses of aspirin may be more suitable for Alzheimer's disease. Ivyspring International Publisher 2020-03-13 /pmc/articles/PMC7085272/ /pubmed/32218705 http://dx.doi.org/10.7150/ijms.40959 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Jorda, Adrian Aldasoro, Martin Aldasoro, Constanza Guerra-Ojeda, Sol Iradi, Antonio Vila, Jose Mª Campos-Campos, Juan Valles, Soraya L. Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture |
title | Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture |
title_full | Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture |
title_fullStr | Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture |
title_full_unstemmed | Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture |
title_short | Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ(1-42) on Astrocytes in primary culture |
title_sort | action of low doses of aspirin in inflammation and oxidative stress induced by aβ(1-42) on astrocytes in primary culture |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085272/ https://www.ncbi.nlm.nih.gov/pubmed/32218705 http://dx.doi.org/10.7150/ijms.40959 |
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