Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

OBJECTIVE: To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ERK1/2 signaling pathway in inducing apoptosis. METHODS: Real-time cellular analysis technology (RTCA) and MTT were used to detect cell proliferation; Annexin V-FITC/PI dua...

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Autores principales: Liu, Jie, He, Lan, Hu, Jing, Li, Kairui, Zhou, Fangliang, Hu, Mei, Luo, Jingjing, Song, Lan, He, Yingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085394/
https://www.ncbi.nlm.nih.gov/pubmed/32215033
http://dx.doi.org/10.1155/2020/2138186
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author Liu, Jie
He, Lan
Hu, Jing
Li, Kairui
Zhou, Fangliang
Hu, Mei
Luo, Jingjing
Song, Lan
He, Yingchun
author_facet Liu, Jie
He, Lan
Hu, Jing
Li, Kairui
Zhou, Fangliang
Hu, Mei
Luo, Jingjing
Song, Lan
He, Yingchun
author_sort Liu, Jie
collection PubMed
description OBJECTIVE: To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ERK1/2 signaling pathway in inducing apoptosis. METHODS: Real-time cellular analysis technology (RTCA) and MTT were used to detect cell proliferation; Annexin V-FITC/PI dual-fluorescence flow cytometry analysis, Hoechst 33342 staining, and mitochondrial membrane potential detection kit were used to detect cell apoptosis; western blot was used to detect protein expression. RESULTS: Different concentrations of isoimperatorin (10 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20  CONCLUSION: Isoimperatorin can induce nasopharyngeal carcinoma CNE2 cell apoptosis through the MAPK/ERK1/2 signaling pathway.
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spelling pubmed-70853942020-03-25 Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway Liu, Jie He, Lan Hu, Jing Li, Kairui Zhou, Fangliang Hu, Mei Luo, Jingjing Song, Lan He, Yingchun Evid Based Complement Alternat Med Research Article OBJECTIVE: To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ERK1/2 signaling pathway in inducing apoptosis. METHODS: Real-time cellular analysis technology (RTCA) and MTT were used to detect cell proliferation; Annexin V-FITC/PI dual-fluorescence flow cytometry analysis, Hoechst 33342 staining, and mitochondrial membrane potential detection kit were used to detect cell apoptosis; western blot was used to detect protein expression. RESULTS: Different concentrations of isoimperatorin (10 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20 μM, 20  CONCLUSION: Isoimperatorin can induce nasopharyngeal carcinoma CNE2 cell apoptosis through the MAPK/ERK1/2 signaling pathway. Hindawi 2020-03-09 /pmc/articles/PMC7085394/ /pubmed/32215033 http://dx.doi.org/10.1155/2020/2138186 Text en Copyright © 2020 Jie Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Jie
He, Lan
Hu, Jing
Li, Kairui
Zhou, Fangliang
Hu, Mei
Luo, Jingjing
Song, Lan
He, Yingchun
Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway
title Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway
title_full Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway
title_fullStr Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway
title_full_unstemmed Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway
title_short Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway
title_sort isoimperatorin induces apoptosis of nasopharyngeal carcinoma cells via the mapk/erk1/2 signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085394/
https://www.ncbi.nlm.nih.gov/pubmed/32215033
http://dx.doi.org/10.1155/2020/2138186
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