Cargando…

A technical review and guide to RNA fluorescence in situ hybridization

RNA-fluorescence in situ hybridization (FISH) is a powerful tool to visualize target messenger RNA transcripts in cultured cells, tissue sections or whole-mount preparations. As the technique has been developed over time, an ever-increasing number of divergent protocols have been published. There is...

Descripción completa

Detalles Bibliográficos
Autores principales:	Young, Alexander P., Jackson, Daniel J., Wyeth, Russell C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	PeerJ Inc. 2020
Materias:	Biochemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085896/ https://www.ncbi.nlm.nih.gov/pubmed/32219032 http://dx.doi.org/10.7717/peerj.8806

Ejemplares similares

Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification
por: Maekiniemi, Anna, et al.
Publicado: (2020)

A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline
por: Yoon, Dong Suk, et al.
Publicado: (2016)

RNA-guided assembly of Rev-RRE nuclear export complexes
por: Bai, Yun, et al.
Publicado: (2014)

Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation
por: Steinert, Hannah, et al.
Publicado: (2017)

Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
por: Ye, Yan, et al.
Publicado: (2022)

Archaeal TFEα/β is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39
por: Blombach, Fabian, et al.
Publicado: (2015)

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
por: Brodl, Eveline, et al.
Publicado: (2018)

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
por: Lee, Clement M.
Publicado: (2016)

2HybridTools, a handy software to facilitate clone identification and mutation mapping from yeast two-hybrid screening
por: Cauchy, Pierre, et al.
Publicado: (2019)

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
por: Baitan, Daniela, et al.
Publicado: (2018)

Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
por: McCoy, Patrick J., et al.
Publicado: (2018)

colocr: an R package for conducting co-localization analysis on fluorescence microscopy images
por: Ahmed, Mahmoud, et al.
Publicado: (2019)

The messy process of guiding proteins into membranes
por: White, Stephen H
Publicado: (2015)

Prediction and characterization of enzymatic activities guided by sequence similarity and genome neighborhood networks
por: Zhao, Suwen, et al.
Publicado: (2014)

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
por: Vincent, Thomas R., et al.
Publicado: (2017)

Minimizing off-target signals in RNA fluorescent in situ hybridization
por: Arvey, Aaron, et al.
Publicado: (2010)

Hybridization of green synthesized silver nanoparticles with poly(ethylene glycol) methacrylate and their biomedical applications
por: Anwar, Natasha, et al.
Publicado: (2022)

Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters
por: Ho, Cheng-Hsun, et al.
Publicado: (2014)

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
por: Nyström, Sofie, et al.
Publicado: (2017)

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
por: Ji, Chen, et al.
Publicado: (2016)

Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
por: Schumacher, Dominik, et al.
Publicado: (2018)

MicroRNA expression in apical periodontitis and pulpal inflammation: a systematic review
por: Al Gashaamy, Zainab Jamal, et al.
Publicado: (2023)

A comprehensive review of circRNA: from purification and identification to disease marker potential
por: Xu, Sheng, et al.
Publicado: (2018)

Cytoplasmic DNA can be detected by RNA fluorescence in situ hybridization
por: Greenberg, Eliraz, et al.
Publicado: (2019)

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
por: Iadevaia, Valentina, et al.
Publicado: (2018)

Chemical structure-guided design of dynapyrazoles, cell-permeable dynein inhibitors with a unique mode of action
por: Steinman, Jonathan B, et al.
Publicado: (2017)

Identification of human phosphoglycerate mutase 1 (PGAM1) inhibitors using hybrid virtual screening approaches
por: Yousaf, Numan, et al.
Publicado: (2023)

Evaluation of two fluorescence immunoassays for the rapid detection of SARS-CoV-2 antigen—new tool to detect infective COVID-19 patients
por: Porte, Lorena, et al.
Publicado: (2021)

Genome mining unearths a hybrid nonribosomal peptide synthetase-like-pteridine synthase biosynthetic gene cluster
por: Park, Hyun Bong, et al.
Publicado: (2017)

Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase
por: Mallam, Anna L, et al.
Publicado: (2014)

Intrinsic disorder within an AKAP-protein kinase A complex guides local substrate phosphorylation
por: Smith, F Donelson, et al.
Publicado: (2013)

Comprehensive analysis of circRNA expression profiles and circRNA-associated competing endogenous RNA networks in IgA nephropathy
por: Liu, Haiyang, et al.
Publicado: (2020)

Allosteric inhibition of a stem cell RNA-binding protein by an intermediary metabolite
por: Clingman, Carina C, et al.
Publicado: (2014)

Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor
por: Kitajima, Nami, et al.
Publicado: (2020)

ALK Gene Rearrangements in Lung Adenocarcinomas: Concordance of Immunohistochemistry, Fluorescence In Situ Hybridization, RNA In Situ Hybridization, and RNA Next-Generation Sequencing Testing
por: Canterbury, Carleigh R., et al.
Publicado: (2021)

qPCR multiplex detection of microRNA and messenger RNA in a single reaction
por: Khoury, Samantha, et al.
Publicado: (2020)

A putative ATPase mediates RNA transcription and capping in a dsRNA virus
por: Yu, Xuekui, et al.
Publicado: (2015)

RNA structures that resist degradation by Xrn1 produce a pathogenic Dengue virus RNA
por: Chapman, Erich G, et al.
Publicado: (2014)

Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
por: Nilsson, Olof Rickard, et al.
Publicado: (2022)

The long non-coding RNA Dali is an epigenetic regulator of neural differentiation
por: Chalei, Vladislava, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_sl1lmvmnc4vlu6dso57u46r77a