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A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue. Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition. Here we demonstrate that in Arab...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086157/ https://www.ncbi.nlm.nih.gov/pubmed/30419294 http://dx.doi.org/10.1016/j.molp.2018.10.009 |
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author | Sánchez-Montesino, Rocío Bouza-Morcillo, Laura Marquez, Julietta Ghita, Melania Duran-Nebreda, Salva Gómez, Luis Holdsworth, Michael J. Bassel, George Oñate-Sánchez, Luis |
author_facet | Sánchez-Montesino, Rocío Bouza-Morcillo, Laura Marquez, Julietta Ghita, Melania Duran-Nebreda, Salva Gómez, Luis Holdsworth, Michael J. Bassel, George Oñate-Sánchez, Luis |
author_sort | Sánchez-Montesino, Rocío |
collection | PubMed |
description | A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue. Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition. Here we demonstrate that in Arabidopsis thaliana seeds, endosperm cell expansion is a key component of germination. Endosperm cells expand to accommodate embryo growth prior to germination. We show that this is an actively regulated process supported by spatiotemporal control of the cell expansion gene EXPANSIN 2 (EXPA2). The NAC transcription factors NAC25 and NAC1L were identified as upstream regulators of EXPA2 expression, gibberellin-mediated endosperm expansion, and seed germination. The DELLA protein RGL2 repressed activation of the EXPA2 promoter by NAC25/NAC1L. Taken together, our findings uncover a key role of the GA/DELLA-NAC25/NAC1L-EXPA2 network in regulating endosperm cell expansion to control the seed-to-seedling transition. |
format | Online Article Text |
id | pubmed-7086157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70861572020-03-25 A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis Sánchez-Montesino, Rocío Bouza-Morcillo, Laura Marquez, Julietta Ghita, Melania Duran-Nebreda, Salva Gómez, Luis Holdsworth, Michael J. Bassel, George Oñate-Sánchez, Luis Mol Plant Article A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue. Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition. Here we demonstrate that in Arabidopsis thaliana seeds, endosperm cell expansion is a key component of germination. Endosperm cells expand to accommodate embryo growth prior to germination. We show that this is an actively regulated process supported by spatiotemporal control of the cell expansion gene EXPANSIN 2 (EXPA2). The NAC transcription factors NAC25 and NAC1L were identified as upstream regulators of EXPA2 expression, gibberellin-mediated endosperm expansion, and seed germination. The DELLA protein RGL2 repressed activation of the EXPA2 promoter by NAC25/NAC1L. Taken together, our findings uncover a key role of the GA/DELLA-NAC25/NAC1L-EXPA2 network in regulating endosperm cell expansion to control the seed-to-seedling transition. Oxford University Press 2019-01-07 /pmc/articles/PMC7086157/ /pubmed/30419294 http://dx.doi.org/10.1016/j.molp.2018.10.009 Text en © 2018 The Author http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Sánchez-Montesino, Rocío Bouza-Morcillo, Laura Marquez, Julietta Ghita, Melania Duran-Nebreda, Salva Gómez, Luis Holdsworth, Michael J. Bassel, George Oñate-Sánchez, Luis A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis |
title | A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis |
title_full | A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis |
title_fullStr | A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis |
title_full_unstemmed | A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis |
title_short | A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis |
title_sort | regulatory module controlling ga-mediated endosperm cell expansion is critical for seed germination in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086157/ https://www.ncbi.nlm.nih.gov/pubmed/30419294 http://dx.doi.org/10.1016/j.molp.2018.10.009 |
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