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A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis

A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue. Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition. Here we demonstrate that in Arab...

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Autores principales: Sánchez-Montesino, Rocío, Bouza-Morcillo, Laura, Marquez, Julietta, Ghita, Melania, Duran-Nebreda, Salva, Gómez, Luis, Holdsworth, Michael J., Bassel, George, Oñate-Sánchez, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086157/
https://www.ncbi.nlm.nih.gov/pubmed/30419294
http://dx.doi.org/10.1016/j.molp.2018.10.009
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author Sánchez-Montesino, Rocío
Bouza-Morcillo, Laura
Marquez, Julietta
Ghita, Melania
Duran-Nebreda, Salva
Gómez, Luis
Holdsworth, Michael J.
Bassel, George
Oñate-Sánchez, Luis
author_facet Sánchez-Montesino, Rocío
Bouza-Morcillo, Laura
Marquez, Julietta
Ghita, Melania
Duran-Nebreda, Salva
Gómez, Luis
Holdsworth, Michael J.
Bassel, George
Oñate-Sánchez, Luis
author_sort Sánchez-Montesino, Rocío
collection PubMed
description A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue. Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition. Here we demonstrate that in Arabidopsis thaliana seeds, endosperm cell expansion is a key component of germination. Endosperm cells expand to accommodate embryo growth prior to germination. We show that this is an actively regulated process supported by spatiotemporal control of the cell expansion gene EXPANSIN 2 (EXPA2). The NAC transcription factors NAC25 and NAC1L were identified as upstream regulators of EXPA2 expression, gibberellin-mediated endosperm expansion, and seed germination. The DELLA protein RGL2 repressed activation of the EXPA2 promoter by NAC25/NAC1L. Taken together, our findings uncover a key role of the GA/DELLA-NAC25/NAC1L-EXPA2 network in regulating endosperm cell expansion to control the seed-to-seedling transition.
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spelling pubmed-70861572020-03-25 A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis Sánchez-Montesino, Rocío Bouza-Morcillo, Laura Marquez, Julietta Ghita, Melania Duran-Nebreda, Salva Gómez, Luis Holdsworth, Michael J. Bassel, George Oñate-Sánchez, Luis Mol Plant Article A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue. Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition. Here we demonstrate that in Arabidopsis thaliana seeds, endosperm cell expansion is a key component of germination. Endosperm cells expand to accommodate embryo growth prior to germination. We show that this is an actively regulated process supported by spatiotemporal control of the cell expansion gene EXPANSIN 2 (EXPA2). The NAC transcription factors NAC25 and NAC1L were identified as upstream regulators of EXPA2 expression, gibberellin-mediated endosperm expansion, and seed germination. The DELLA protein RGL2 repressed activation of the EXPA2 promoter by NAC25/NAC1L. Taken together, our findings uncover a key role of the GA/DELLA-NAC25/NAC1L-EXPA2 network in regulating endosperm cell expansion to control the seed-to-seedling transition. Oxford University Press 2019-01-07 /pmc/articles/PMC7086157/ /pubmed/30419294 http://dx.doi.org/10.1016/j.molp.2018.10.009 Text en © 2018 The Author http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Sánchez-Montesino, Rocío
Bouza-Morcillo, Laura
Marquez, Julietta
Ghita, Melania
Duran-Nebreda, Salva
Gómez, Luis
Holdsworth, Michael J.
Bassel, George
Oñate-Sánchez, Luis
A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
title A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
title_full A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
title_fullStr A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
title_full_unstemmed A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
title_short A Regulatory Module Controlling GA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
title_sort regulatory module controlling ga-mediated endosperm cell expansion is critical for seed germination in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086157/
https://www.ncbi.nlm.nih.gov/pubmed/30419294
http://dx.doi.org/10.1016/j.molp.2018.10.009
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