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MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10

Nasopharyngeal carcinoma (NPC), is one of the most common malignant tumor in southern China and southeast Asia. MYH10 is a coding gene of the NMMHC-IIB protein. Previous studies have shown that MYH10 expression was up-regulated in breast cancer, glioma and meningioma. Moreover, it was targeted by mi...

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Autores principales: Liu, Wenya, Cai, Tonghui, Li, Lingjun, Chen, Hui, Chen, Ruichao, Zhang, Minfen, Zhang, Wei, Zhao, Li, Xiong, Hanzhen, Qin, Ping, Gao, Xingcheng, Jiang, Qingping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086266/
https://www.ncbi.nlm.nih.gov/pubmed/32226520
http://dx.doi.org/10.7150/jca.40438
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author Liu, Wenya
Cai, Tonghui
Li, Lingjun
Chen, Hui
Chen, Ruichao
Zhang, Minfen
Zhang, Wei
Zhao, Li
Xiong, Hanzhen
Qin, Ping
Gao, Xingcheng
Jiang, Qingping
author_facet Liu, Wenya
Cai, Tonghui
Li, Lingjun
Chen, Hui
Chen, Ruichao
Zhang, Minfen
Zhang, Wei
Zhao, Li
Xiong, Hanzhen
Qin, Ping
Gao, Xingcheng
Jiang, Qingping
author_sort Liu, Wenya
collection PubMed
description Nasopharyngeal carcinoma (NPC), is one of the most common malignant tumor in southern China and southeast Asia. MYH10 is a coding gene of the NMMHC-IIB protein. Previous studies have shown that MYH10 expression was up-regulated in breast cancer, glioma and meningioma. Moreover, it was targeted by miR200 family. However, no relevant studies have been found in NPC. In present study, we found in 48 NPC specimens, MYH10 level was lower in most cancer areas than that in the adjacent normal tissue. Moreover, the depletion of MYH10 can promote the migration and invasion of NPC. In addition, we demonstrated that miR-200a has the strongest regulation to MYH10 among miR-200 family. miR-200a mimics could decrease MYH10 expression, while miR-200a inhibitor increase MYH10 expression. Next, we found that miR-200a bound directly to MYH10 using Dual-luciferase reporter. Finally, it was demonstrated that siMYH10 could reverse the effect of miR-200a inhibitor on NPC cell migration and invasion. Taken together, it can be concluded that MYH10 is lowly expressed in NPC compared with adjacent tissues, and the loss of MYH10 can promote the migration and invasion of NPC cells; Among the miR-200 family, miR-200a has the strongest regulatory effect on MYH10; MYH10 is a direct target gene of miR200a, and miR200a targets MYH10 to regulate the migration and invasion of NPC cells.
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spelling pubmed-70862662020-03-27 MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10 Liu, Wenya Cai, Tonghui Li, Lingjun Chen, Hui Chen, Ruichao Zhang, Minfen Zhang, Wei Zhao, Li Xiong, Hanzhen Qin, Ping Gao, Xingcheng Jiang, Qingping J Cancer Research Paper Nasopharyngeal carcinoma (NPC), is one of the most common malignant tumor in southern China and southeast Asia. MYH10 is a coding gene of the NMMHC-IIB protein. Previous studies have shown that MYH10 expression was up-regulated in breast cancer, glioma and meningioma. Moreover, it was targeted by miR200 family. However, no relevant studies have been found in NPC. In present study, we found in 48 NPC specimens, MYH10 level was lower in most cancer areas than that in the adjacent normal tissue. Moreover, the depletion of MYH10 can promote the migration and invasion of NPC. In addition, we demonstrated that miR-200a has the strongest regulation to MYH10 among miR-200 family. miR-200a mimics could decrease MYH10 expression, while miR-200a inhibitor increase MYH10 expression. Next, we found that miR-200a bound directly to MYH10 using Dual-luciferase reporter. Finally, it was demonstrated that siMYH10 could reverse the effect of miR-200a inhibitor on NPC cell migration and invasion. Taken together, it can be concluded that MYH10 is lowly expressed in NPC compared with adjacent tissues, and the loss of MYH10 can promote the migration and invasion of NPC cells; Among the miR-200 family, miR-200a has the strongest regulatory effect on MYH10; MYH10 is a direct target gene of miR200a, and miR200a targets MYH10 to regulate the migration and invasion of NPC cells. Ivyspring International Publisher 2020-03-04 /pmc/articles/PMC7086266/ /pubmed/32226520 http://dx.doi.org/10.7150/jca.40438 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Liu, Wenya
Cai, Tonghui
Li, Lingjun
Chen, Hui
Chen, Ruichao
Zhang, Minfen
Zhang, Wei
Zhao, Li
Xiong, Hanzhen
Qin, Ping
Gao, Xingcheng
Jiang, Qingping
MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
title MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
title_full MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
title_fullStr MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
title_full_unstemmed MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
title_short MiR-200a Regulates Nasopharyngeal Carcinoma Cell Migration and Invasion by Targeting MYH10
title_sort mir-200a regulates nasopharyngeal carcinoma cell migration and invasion by targeting myh10
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086266/
https://www.ncbi.nlm.nih.gov/pubmed/32226520
http://dx.doi.org/10.7150/jca.40438
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