pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli

Glutathione-S-transferase (GST) fusion protein expression vectors are often employed for the expression and purification of proteins in Escherichia coli. GST is then removed by site-specific proteolysis using thrombin. However, the presence of internal thrombin cleavage sites in expressed proteins c...

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Detalles Bibliográficos
Autores principales: Purbey, Prabhat Kumar, Jayakumar, P Cyril, Patole, Milind S, Galande, Sanjeev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086577/
https://www.ncbi.nlm.nih.gov/pubmed/17487164
http://dx.doi.org/10.1038/nprot.2006.310

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086577/
https://www.ncbi.nlm.nih.gov/pubmed/17487164
http://dx.doi.org/10.1038/nprot.2006.310

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