Molecular characterization and phylogenetic analysis of the genome of porcine torovirus

In this study, we amplified and sequenced the first genome of porcine torovirus (PToV SH1 strain). The genome was found to be 28,301 bp in length, sharing 79 % identity with Breda virus. It mainly consists of replicase (20,906 bp) and structural genes: spike (4,722 bp), membrane (702 bp), hemaggluti...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Huan, Lan, Daoliang, Lu, Lifang, Chen, Molin, Wang, Changsong, Hua, Xiuguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2013
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086580/
https://www.ncbi.nlm.nih.gov/pubmed/24122107
http://dx.doi.org/10.1007/s00705-013-1861-x
_version_ 1783509150228021248
author Sun, Huan
Lan, Daoliang
Lu, Lifang
Chen, Molin
Wang, Changsong
Hua, Xiuguo
author_facet Sun, Huan
Lan, Daoliang
Lu, Lifang
Chen, Molin
Wang, Changsong
Hua, Xiuguo
author_sort Sun, Huan
collection PubMed
description In this study, we amplified and sequenced the first genome of porcine torovirus (PToV SH1 strain). The genome was found to be 28,301 bp in length, sharing 79 % identity with Breda virus. It mainly consists of replicase (20,906 bp) and structural genes: spike (4,722 bp), membrane (702 bp), hemagglutinin-esterase (1,284 bp), and nucleocapsid (492 bp). Sequence alignments and structure prediction suggest genetic differences among toroviruses, mainly in NSP1 (papain-like cysteine proteinase domain). Rooted phylogenetic trees were constructed based on the 3C-like proteinase and RNA-dependent RNA polymerase genes. PToV, Berne virus and Breda virus were clustered together, forming a separate branch from white bream virus that was distant from that of the coronaviruses.
format Online
Article
Text
id pubmed-7086580
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-70865802020-03-23 Molecular characterization and phylogenetic analysis of the genome of porcine torovirus Sun, Huan Lan, Daoliang Lu, Lifang Chen, Molin Wang, Changsong Hua, Xiuguo Arch Virol Brief Report In this study, we amplified and sequenced the first genome of porcine torovirus (PToV SH1 strain). The genome was found to be 28,301 bp in length, sharing 79 % identity with Breda virus. It mainly consists of replicase (20,906 bp) and structural genes: spike (4,722 bp), membrane (702 bp), hemagglutinin-esterase (1,284 bp), and nucleocapsid (492 bp). Sequence alignments and structure prediction suggest genetic differences among toroviruses, mainly in NSP1 (papain-like cysteine proteinase domain). Rooted phylogenetic trees were constructed based on the 3C-like proteinase and RNA-dependent RNA polymerase genes. PToV, Berne virus and Breda virus were clustered together, forming a separate branch from white bream virus that was distant from that of the coronaviruses. Springer Vienna 2013-10-12 2014 /pmc/articles/PMC7086580/ /pubmed/24122107 http://dx.doi.org/10.1007/s00705-013-1861-x Text en © Springer-Verlag Wien 2013 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Sun, Huan
Lan, Daoliang
Lu, Lifang
Chen, Molin
Wang, Changsong
Hua, Xiuguo
Molecular characterization and phylogenetic analysis of the genome of porcine torovirus
title Molecular characterization and phylogenetic analysis of the genome of porcine torovirus
title_full Molecular characterization and phylogenetic analysis of the genome of porcine torovirus
title_fullStr Molecular characterization and phylogenetic analysis of the genome of porcine torovirus
title_full_unstemmed Molecular characterization and phylogenetic analysis of the genome of porcine torovirus
title_short Molecular characterization and phylogenetic analysis of the genome of porcine torovirus
title_sort molecular characterization and phylogenetic analysis of the genome of porcine torovirus
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086580/
https://www.ncbi.nlm.nih.gov/pubmed/24122107
http://dx.doi.org/10.1007/s00705-013-1861-x
work_keys_str_mv AT sunhuan molecularcharacterizationandphylogeneticanalysisofthegenomeofporcinetorovirus
AT landaoliang molecularcharacterizationandphylogeneticanalysisofthegenomeofporcinetorovirus
AT lulifang molecularcharacterizationandphylogeneticanalysisofthegenomeofporcinetorovirus
AT chenmolin molecularcharacterizationandphylogeneticanalysisofthegenomeofporcinetorovirus
AT wangchangsong molecularcharacterizationandphylogeneticanalysisofthegenomeofporcinetorovirus
AT huaxiuguo molecularcharacterizationandphylogeneticanalysisofthegenomeofporcinetorovirus

Ejemplares similares