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Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus

 Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), conta...

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Autores principales: Gómez, N., Wigdorovitz, A., Castañón, S., Gil, F., Ordá, R., Borca, M. V., Escribano, J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086604/
https://www.ncbi.nlm.nih.gov/pubmed/11003480
http://dx.doi.org/10.1007/s007050070087
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author Gómez, N.
Wigdorovitz, A.
Castañón, S.
Gil, F.
Ordá, R.
Borca, M. V.
Escribano, J. M.
author_facet Gómez, N.
Wigdorovitz, A.
Castañón, S.
Gil, F.
Ordá, R.
Borca, M. V.
Escribano, J. M.
author_sort Gómez, N.
collection PubMed
description  Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.
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spelling pubmed-70866042020-03-23 Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus Gómez, N. Wigdorovitz, A. Castañón, S. Gil, F. Ordá, R. Borca, M. V. Escribano, J. M. Arch Virol Brief Report  Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV. Springer-Verlag 2000 /pmc/articles/PMC7086604/ /pubmed/11003480 http://dx.doi.org/10.1007/s007050070087 Text en © Springer-Verlag/Wien 2000 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Gómez, N.
Wigdorovitz, A.
Castañón, S.
Gil, F.
Ordá, R.
Borca, M. V.
Escribano, J. M.
Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_full Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_fullStr Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_full_unstemmed Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_short Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
title_sort oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086604/
https://www.ncbi.nlm.nih.gov/pubmed/11003480
http://dx.doi.org/10.1007/s007050070087
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