Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus

 The aim of this study was to identify the receptor(s) for PRRSV on porcine alveolar macrophages (PAMs) by producing monoclonal antibodies (MAbs) against these cells. Hybridoma supernatants were selected for their ability to block PRRSV infection. Four MAbs, 1-8D2, 9.4C7, 9.9F2, and 3-3H2 inhibited...

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Autores principales: Wissink, E. H. J., van Wijk, H. A. R., Pol, J. M. A., Godeke, G.-J., van Rijn, P. A., Rottier, P. J. M., Meulenberg, J. J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2003
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086606/
https://www.ncbi.nlm.nih.gov/pubmed/12536303
http://dx.doi.org/10.1007/s00705-002-0897-0
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author Wissink, E. H. J.
van Wijk, H. A. R.
Pol, J. M. A.
Godeke, G.-J.
van Rijn, P. A.
Rottier, P. J. M.
Meulenberg, J. J. M.
author_facet Wissink, E. H. J.
van Wijk, H. A. R.
Pol, J. M. A.
Godeke, G.-J.
van Rijn, P. A.
Rottier, P. J. M.
Meulenberg, J. J. M.
author_sort Wissink, E. H. J.
collection PubMed
description  The aim of this study was to identify the receptor(s) for PRRSV on porcine alveolar macrophages (PAMs) by producing monoclonal antibodies (MAbs) against these cells. Hybridoma supernatants were selected for their ability to block PRRSV infection. Four MAbs, 1-8D2, 9.4C7, 9.9F2, and 3-3H2 inhibited infection and recognised cell surface, PAM-specific antigens as shown by immunofluorescence and immunoperoxidase monolayer assay. These MAbs were then used to identify cellular proteins involved in PRRSV infection by radioimmunoprecipitation assays (RIPAs). MAbs 1-8D2 and 9.9F2 each recognised a 150 kDa-polypeptide doublet, while MAbs 9.4C7 and 3-3H2 both recognised a 220 kDa-polypeptide. Glycosidase treatment demonstrated all these polypeptides to be N-glycosylated. Thus, multiple glycoproteins appear to be involved in infection of PAMs by PRRSV.
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spelling pubmed-70866062020-03-23 Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus Wissink, E. H. J. van Wijk, H. A. R. Pol, J. M. A. Godeke, G.-J. van Rijn, P. A. Rottier, P. J. M. Meulenberg, J. J. M. Arch Virol Brief Report  The aim of this study was to identify the receptor(s) for PRRSV on porcine alveolar macrophages (PAMs) by producing monoclonal antibodies (MAbs) against these cells. Hybridoma supernatants were selected for their ability to block PRRSV infection. Four MAbs, 1-8D2, 9.4C7, 9.9F2, and 3-3H2 inhibited infection and recognised cell surface, PAM-specific antigens as shown by immunofluorescence and immunoperoxidase monolayer assay. These MAbs were then used to identify cellular proteins involved in PRRSV infection by radioimmunoprecipitation assays (RIPAs). MAbs 1-8D2 and 9.9F2 each recognised a 150 kDa-polypeptide doublet, while MAbs 9.4C7 and 3-3H2 both recognised a 220 kDa-polypeptide. Glycosidase treatment demonstrated all these polypeptides to be N-glycosylated. Thus, multiple glycoproteins appear to be involved in infection of PAMs by PRRSV. Springer-Verlag 2003 /pmc/articles/PMC7086606/ /pubmed/12536303 http://dx.doi.org/10.1007/s00705-002-0897-0 Text en © Springer-Verlag/Wien 2003 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Wissink, E. H. J.
van Wijk, H. A. R.
Pol, J. M. A.
Godeke, G.-J.
van Rijn, P. A.
Rottier, P. J. M.
Meulenberg, J. J. M.
Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus
title Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus
title_full Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus
title_fullStr Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus
title_full_unstemmed Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus
title_short Identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus
title_sort identification of porcine alveolar macrophage glycoproteins involved in infection of porcine respiratory and reproductive syndrome virus
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086606/
https://www.ncbi.nlm.nih.gov/pubmed/12536303
http://dx.doi.org/10.1007/s00705-002-0897-0
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