Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults

In this study, we investigated turkey reovirus (TReoV) in tissue samples from young birds, aged 15 days. RT-PCR for TReoV detected 3.3 % positive samples and TReoV was successfully isolated in Vero cells. Histological analysis of positive bursa of Fabricius (BF) revealed atrophied follicles and lymp...

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Autores principales: Rosa, Ana Carolina G., Ferreira, Helena Lage, Gomes, Deriane Elias, Táparo, Cilene Vidovix, Cardoso, Tereza Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2013
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086608/
https://www.ncbi.nlm.nih.gov/pubmed/24327096
http://dx.doi.org/10.1007/s00705-013-1947-5
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author Rosa, Ana Carolina G.
Ferreira, Helena Lage
Gomes, Deriane Elias
Táparo, Cilene Vidovix
Cardoso, Tereza Cristina
author_facet Rosa, Ana Carolina G.
Ferreira, Helena Lage
Gomes, Deriane Elias
Táparo, Cilene Vidovix
Cardoso, Tereza Cristina
author_sort Rosa, Ana Carolina G.
collection PubMed
description In this study, we investigated turkey reovirus (TReoV) in tissue samples from young birds, aged 15 days. RT-PCR for TReoV detected 3.3 % positive samples and TReoV was successfully isolated in Vero cells. Histological analysis of positive bursa of Fabricius (BF) revealed atrophied follicles and lymphocyte depletion. The number of CD8+, CD4+ and IgM+ cells was lower in infected BF. Phylogenetic analysis based on S3 gene showed that the Brazilian TReoV isolates clustered in a single group with 98-100 % similarity to TReoV strains circulating in the United States. This is the first indication that TReoV infection may be a contributing factor to immunosuppression in young birds.
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spelling pubmed-70866082020-03-23 Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults Rosa, Ana Carolina G. Ferreira, Helena Lage Gomes, Deriane Elias Táparo, Cilene Vidovix Cardoso, Tereza Cristina Arch Virol Brief Report In this study, we investigated turkey reovirus (TReoV) in tissue samples from young birds, aged 15 days. RT-PCR for TReoV detected 3.3 % positive samples and TReoV was successfully isolated in Vero cells. Histological analysis of positive bursa of Fabricius (BF) revealed atrophied follicles and lymphocyte depletion. The number of CD8+, CD4+ and IgM+ cells was lower in infected BF. Phylogenetic analysis based on S3 gene showed that the Brazilian TReoV isolates clustered in a single group with 98-100 % similarity to TReoV strains circulating in the United States. This is the first indication that TReoV infection may be a contributing factor to immunosuppression in young birds. Springer Vienna 2013-12-11 2014 /pmc/articles/PMC7086608/ /pubmed/24327096 http://dx.doi.org/10.1007/s00705-013-1947-5 Text en © Springer-Verlag Wien 2013 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Rosa, Ana Carolina G.
Ferreira, Helena Lage
Gomes, Deriane Elias
Táparo, Cilene Vidovix
Cardoso, Tereza Cristina
Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults
title Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults
title_full Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults
title_fullStr Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults
title_full_unstemmed Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults
title_short Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults
title_sort isolation and molecular characterization of brazilian turkey reovirus from immunosuppressed young poults
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086608/
https://www.ncbi.nlm.nih.gov/pubmed/24327096
http://dx.doi.org/10.1007/s00705-013-1947-5
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