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Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus

 To group infectious bronchitis virus (IBV) isolates, a genetic grouping method based on hypervariable region 1 (HVR 1, nucleotides 168 to 197) was compared with that based on the whole S1 gene. Both methods resulted in the same grouping data. So the grouping method based on HVR 1 could represent th...

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Detalles Bibliográficos
Autores principales: Wang, C. -H., Huang, Y. -C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086635/
https://www.ncbi.nlm.nih.gov/pubmed/10752554
http://dx.doi.org/10.1007/s007050050024
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author Wang, C. -H.
Huang, Y. -C.
author_facet Wang, C. -H.
Huang, Y. -C.
author_sort Wang, C. -H.
collection PubMed
description  To group infectious bronchitis virus (IBV) isolates, a genetic grouping method based on hypervariable region 1 (HVR 1, nucleotides 168 to 197) was compared with that based on the whole S1 gene. Both methods resulted in the same grouping data. So the grouping method based on HVR 1 could represent the grouping method based on the whole S1 gene. Taiwan isolates could not be placed within the existing groups. In order to test the correlation between genotype and serotype, a one-way neutralization test was used to compare 9 Taiwan isolates selected from different genotypes with Massachusetts (Mass) (H120) and Connecticut (Conn) standard strains. In addition, a two-way cross-neutralization test was performed in embryonated eggs with the β method (constant-virus, diluted-serum) and the reciprocal neutralization titers were calculated to give the relatedness (r) values. The results of two kinds of neutralizing tests showed that the serotypes of 9 isolates were different from Mass or Conn. Based on the r-values, 9 isolates were divided into two serotypes which were correlated with their genotypes. From pathogenicity tests, IBV Taiwan isolates could be divided into high, intermediate, and low pathogenicity according to their pathogenicity indexes. However, no relationship exists between pathotype and genotype. In conclusion, the genetic typing method based on HVR 1 can be used for typing IBV isolates.
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spelling pubmed-70866352020-03-23 Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus Wang, C. -H. Huang, Y. -C. Arch Virol Article  To group infectious bronchitis virus (IBV) isolates, a genetic grouping method based on hypervariable region 1 (HVR 1, nucleotides 168 to 197) was compared with that based on the whole S1 gene. Both methods resulted in the same grouping data. So the grouping method based on HVR 1 could represent the grouping method based on the whole S1 gene. Taiwan isolates could not be placed within the existing groups. In order to test the correlation between genotype and serotype, a one-way neutralization test was used to compare 9 Taiwan isolates selected from different genotypes with Massachusetts (Mass) (H120) and Connecticut (Conn) standard strains. In addition, a two-way cross-neutralization test was performed in embryonated eggs with the β method (constant-virus, diluted-serum) and the reciprocal neutralization titers were calculated to give the relatedness (r) values. The results of two kinds of neutralizing tests showed that the serotypes of 9 isolates were different from Mass or Conn. Based on the r-values, 9 isolates were divided into two serotypes which were correlated with their genotypes. From pathogenicity tests, IBV Taiwan isolates could be divided into high, intermediate, and low pathogenicity according to their pathogenicity indexes. However, no relationship exists between pathotype and genotype. In conclusion, the genetic typing method based on HVR 1 can be used for typing IBV isolates. Springer-Verlag 2000 /pmc/articles/PMC7086635/ /pubmed/10752554 http://dx.doi.org/10.1007/s007050050024 Text en © Springer-Verlag/Wien 2000 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Wang, C. -H.
Huang, Y. -C.
Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus
title Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus
title_full Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus
title_fullStr Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus
title_full_unstemmed Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus
title_short Relationship between serotypes and genotypes based on the hypervariable region of the S1 gene of infectious bronchitis virus
title_sort relationship between serotypes and genotypes based on the hypervariable region of the s1 gene of infectious bronchitis virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086635/
https://www.ncbi.nlm.nih.gov/pubmed/10752554
http://dx.doi.org/10.1007/s007050050024
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