Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody

Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to ne...

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Detalles Bibliográficos
Autores principales: Lecomte, J., Cainelli-Gebara, V., Mercier, G., Mansour, S., Talbot, P. J., Lussier, G., Oth, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1987
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086664/
https://www.ncbi.nlm.nih.gov/pubmed/2825619
http://dx.doi.org/10.1007/BF01310740
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author Lecomte, J.
Cainelli-Gebara, V.
Mercier, G.
Mansour, S.
Talbot, P. J.
Lussier, G.
Oth, D.
author_facet Lecomte, J.
Cainelli-Gebara, V.
Mercier, G.
Mansour, S.
Talbot, P. J.
Lussier, G.
Oth, D.
author_sort Lecomte, J.
collection PubMed
description Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immuno-fluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.
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spelling pubmed-70866642020-03-23 Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody Lecomte, J. Cainelli-Gebara, V. Mercier, G. Mansour, S. Talbot, P. J. Lussier, G. Oth, D. Arch Virol Brief Report Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immuno-fluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3. Springer-Verlag 1987 /pmc/articles/PMC7086664/ /pubmed/2825619 http://dx.doi.org/10.1007/BF01310740 Text en © by Springer-Verlag 1987 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Lecomte, J.
Cainelli-Gebara, V.
Mercier, G.
Mansour, S.
Talbot, P. J.
Lussier, G.
Oth, D.
Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
title Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
title_full Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
title_fullStr Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
title_full_unstemmed Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
title_short Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
title_sort protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086664/
https://www.ncbi.nlm.nih.gov/pubmed/2825619
http://dx.doi.org/10.1007/BF01310740
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