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Experimental infection of adult axenic rats with Parker's Rat Coronavirus

The pathogenesis of Parker's Rat Coronavirus (PRCV) was studied in axenic CD rats. Three to four 9 to 10 week old rats were euthanized daily for eight days after intranasal inoculation. Rats remained free of clinical disease. Virus was recovered from the nasopharynx and trachea after twenty-fou...

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Detalles Bibliográficos
Autores principales: Bhatt, P. N., Jacoby, R. O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1977
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086747/
https://www.ncbi.nlm.nih.gov/pubmed/907478
http://dx.doi.org/10.1007/BF01314779
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author Bhatt, P. N.
Jacoby, R. O.
author_facet Bhatt, P. N.
Jacoby, R. O.
author_sort Bhatt, P. N.
collection PubMed
description The pathogenesis of Parker's Rat Coronavirus (PRCV) was studied in axenic CD rats. Three to four 9 to 10 week old rats were euthanized daily for eight days after intranasal inoculation. Rats remained free of clinical disease. Virus was recovered from the nasopharynx and trachea after twenty-four hours and from the lung by day three but was not detected in respiratory tract after seven days. Viral antigen was detected by indirect immunofluorescence in the mucosal epithelium of upper respiratory tract and in pulmonary alveolar septae from day two to six postinoculation. Acute rhinitis developed by day two and was associated with mild focal necrosis of respiratory mucosal epithelium. Mild nonsuppurative tracheitis and multifocal interstitial pneumonia appeared by day five and persisted through day eight. Dacryoadenitis did not occur, sialoadenitis was detected in only three rats and virus was recovered from only one submaxillary salivary gland. This experiment indicates that PRCV can be a primary pathogen for the respiratory system of adult rats. In contrast to sialodacryoadenitis (SDA) virus the tropism of PRCV for salivary and lacrimal glands is low.
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spelling pubmed-70867472020-03-23 Experimental infection of adult axenic rats with Parker's Rat Coronavirus Bhatt, P. N. Jacoby, R. O. Arch Virol Original Papers The pathogenesis of Parker's Rat Coronavirus (PRCV) was studied in axenic CD rats. Three to four 9 to 10 week old rats were euthanized daily for eight days after intranasal inoculation. Rats remained free of clinical disease. Virus was recovered from the nasopharynx and trachea after twenty-four hours and from the lung by day three but was not detected in respiratory tract after seven days. Viral antigen was detected by indirect immunofluorescence in the mucosal epithelium of upper respiratory tract and in pulmonary alveolar septae from day two to six postinoculation. Acute rhinitis developed by day two and was associated with mild focal necrosis of respiratory mucosal epithelium. Mild nonsuppurative tracheitis and multifocal interstitial pneumonia appeared by day five and persisted through day eight. Dacryoadenitis did not occur, sialoadenitis was detected in only three rats and virus was recovered from only one submaxillary salivary gland. This experiment indicates that PRCV can be a primary pathogen for the respiratory system of adult rats. In contrast to sialodacryoadenitis (SDA) virus the tropism of PRCV for salivary and lacrimal glands is low. Springer-Verlag 1977 /pmc/articles/PMC7086747/ /pubmed/907478 http://dx.doi.org/10.1007/BF01314779 Text en © Springer-Verlag 1977 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Papers
Bhatt, P. N.
Jacoby, R. O.
Experimental infection of adult axenic rats with Parker's Rat Coronavirus
title Experimental infection of adult axenic rats with Parker's Rat Coronavirus
title_full Experimental infection of adult axenic rats with Parker's Rat Coronavirus
title_fullStr Experimental infection of adult axenic rats with Parker's Rat Coronavirus
title_full_unstemmed Experimental infection of adult axenic rats with Parker's Rat Coronavirus
title_short Experimental infection of adult axenic rats with Parker's Rat Coronavirus
title_sort experimental infection of adult axenic rats with parker's rat coronavirus
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086747/
https://www.ncbi.nlm.nih.gov/pubmed/907478
http://dx.doi.org/10.1007/BF01314779
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