Characterization of equine arteritis virus particles and demonstration of their hemolytic activity

Equine arteritis virus (EAV), a member of the newly established family Arteriviridae, is a small, positive-stranded RNA virus. It carries two protein complexes in its envelope, gp5/M and the recently described gp2b/gp3/gp4 complex. We report here on several basic features of EAV replication in cell...

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Detalles Bibliográficos
Autores principales: Veit, M., Kabatek, A., Tielesch, C., Hermann, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2008
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086761/
https://www.ncbi.nlm.nih.gov/pubmed/18219439
http://dx.doi.org/10.1007/s00705-007-1094-y
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author Veit, M.
Kabatek, A.
Tielesch, C.
Hermann, A.
author_facet Veit, M.
Kabatek, A.
Tielesch, C.
Hermann, A.
author_sort Veit, M.
collection PubMed
description Equine arteritis virus (EAV), a member of the newly established family Arteriviridae, is a small, positive-stranded RNA virus. It carries two protein complexes in its envelope, gp5/M and the recently described gp2b/gp3/gp4 complex. We report here on several basic features of EAV replication in cell culture and on the protein composition of virus particles. We have also characterized gp2b, gp3, and gp4 expressed using a baculovirus system in insect cells. Finally, we provide evidence that EAV possess hemagglutinating and hemolytic activity. The hemolysis assay might be useful for determining which of the surface proteins carries the receptor-binding and membrane fusion activity of EAV.
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spelling pubmed-70867612020-03-23 Characterization of equine arteritis virus particles and demonstration of their hemolytic activity Veit, M. Kabatek, A. Tielesch, C. Hermann, A. Arch Virol Brief Report Equine arteritis virus (EAV), a member of the newly established family Arteriviridae, is a small, positive-stranded RNA virus. It carries two protein complexes in its envelope, gp5/M and the recently described gp2b/gp3/gp4 complex. We report here on several basic features of EAV replication in cell culture and on the protein composition of virus particles. We have also characterized gp2b, gp3, and gp4 expressed using a baculovirus system in insect cells. Finally, we provide evidence that EAV possess hemagglutinating and hemolytic activity. The hemolysis assay might be useful for determining which of the surface proteins carries the receptor-binding and membrane fusion activity of EAV. Springer-Verlag 2008-02-04 2008 /pmc/articles/PMC7086761/ /pubmed/18219439 http://dx.doi.org/10.1007/s00705-007-1094-y Text en © Springer-Verlag 2008 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Veit, M.
Kabatek, A.
Tielesch, C.
Hermann, A.
Characterization of equine arteritis virus particles and demonstration of their hemolytic activity
title Characterization of equine arteritis virus particles and demonstration of their hemolytic activity
title_full Characterization of equine arteritis virus particles and demonstration of their hemolytic activity
title_fullStr Characterization of equine arteritis virus particles and demonstration of their hemolytic activity
title_full_unstemmed Characterization of equine arteritis virus particles and demonstration of their hemolytic activity
title_short Characterization of equine arteritis virus particles and demonstration of their hemolytic activity
title_sort characterization of equine arteritis virus particles and demonstration of their hemolytic activity
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086761/
https://www.ncbi.nlm.nih.gov/pubmed/18219439
http://dx.doi.org/10.1007/s00705-007-1094-y
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AT tieleschc characterizationofequinearteritisvirusparticlesanddemonstrationoftheirhemolyticactivity
AT hermanna characterizationofequinearteritisvirusparticlesanddemonstrationoftheirhemolyticactivity

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