Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus

Little is known about the neutralizing epitopes in turkey coronavirus (TCoV). The spike (S) protein gene of TCoV was divided into 10 fragments to identify the antigenic region containing neutralizing epitopes. The expression and antigenicity of S fragments was confirmed by immunofluorescence antibod...

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Detalles Bibliográficos
Autores principales: Chen, Yi-Ning, Wu, Ching Ching, Lin, Tsang Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2011
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086772/
https://www.ncbi.nlm.nih.gov/pubmed/21594597
http://dx.doi.org/10.1007/s00705-011-1020-1
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author Chen, Yi-Ning
Wu, Ching Ching
Lin, Tsang Long
author_facet Chen, Yi-Ning
Wu, Ching Ching
Lin, Tsang Long
author_sort Chen, Yi-Ning
collection PubMed
description Little is known about the neutralizing epitopes in turkey coronavirus (TCoV). The spike (S) protein gene of TCoV was divided into 10 fragments to identify the antigenic region containing neutralizing epitopes. The expression and antigenicity of S fragments was confirmed by immunofluorescence antibody (IFA) assay using an anti-histidine monoclonal antibody or anti-TCoV serum. Polyclonal antibodies raised against expressed S1 (amino acid position 1 to 573 from start codon of S protein), 4F/4R (482-678), 6F/6R (830-1071), or Mod4F/Epi4R (476-520) S fragment recognized native S1 protein and TCoV in the intestines of TCoV-infected turkey embryos. Anti-TCoV serum reacted with recombinant 4F/4R, 6F/6R, and Mod4F/Epi4R in a western blot. The results of a virus neutralization assay indicated that the carboxyl terminal region of the S1 protein (Mod4F/Epi4R) or the combined carboxyl terminal S1 and amino terminal S2 protein (4F/4R) possesses the neutralizing epitopes, while the S2 fragment (6F/6R) contains antigenic epitopes but not neutralizing epitopes.
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spelling pubmed-70867722020-03-23 Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus Chen, Yi-Ning Wu, Ching Ching Lin, Tsang Long Arch Virol Original Article Little is known about the neutralizing epitopes in turkey coronavirus (TCoV). The spike (S) protein gene of TCoV was divided into 10 fragments to identify the antigenic region containing neutralizing epitopes. The expression and antigenicity of S fragments was confirmed by immunofluorescence antibody (IFA) assay using an anti-histidine monoclonal antibody or anti-TCoV serum. Polyclonal antibodies raised against expressed S1 (amino acid position 1 to 573 from start codon of S protein), 4F/4R (482-678), 6F/6R (830-1071), or Mod4F/Epi4R (476-520) S fragment recognized native S1 protein and TCoV in the intestines of TCoV-infected turkey embryos. Anti-TCoV serum reacted with recombinant 4F/4R, 6F/6R, and Mod4F/Epi4R in a western blot. The results of a virus neutralization assay indicated that the carboxyl terminal region of the S1 protein (Mod4F/Epi4R) or the combined carboxyl terminal S1 and amino terminal S2 protein (4F/4R) possesses the neutralizing epitopes, while the S2 fragment (6F/6R) contains antigenic epitopes but not neutralizing epitopes. Springer Vienna 2011-05-19 2011 /pmc/articles/PMC7086772/ /pubmed/21594597 http://dx.doi.org/10.1007/s00705-011-1020-1 Text en © Springer-Verlag 2011 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Chen, Yi-Ning
Wu, Ching Ching
Lin, Tsang Long
Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus
title Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus
title_full Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus
title_fullStr Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus
title_full_unstemmed Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus
title_short Identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus
title_sort identification and characterization of a neutralizing-epitope-containing spike protein fragment in turkey coronavirus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086772/
https://www.ncbi.nlm.nih.gov/pubmed/21594597
http://dx.doi.org/10.1007/s00705-011-1020-1
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