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Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2
It has been reported that dual or multiple viruses can coinfect epithelial cells of the respiratory tract. However, little has been reported on in vitro interactions of coinfected viruses. To explore how coinfection of different viruses affects their biological property, we examined growth of influe...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086786/ https://www.ncbi.nlm.nih.gov/pubmed/26582554 http://dx.doi.org/10.1007/s00430-015-0441-y |
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author | Goto, Hideo Ihira, Hironobu Morishita, Keiichi Tsuchiya, Mitsuki Ohta, Keisuke Yumine, Natsuko Tsurudome, Masato Nishio, Machiko |
author_facet | Goto, Hideo Ihira, Hironobu Morishita, Keiichi Tsuchiya, Mitsuki Ohta, Keisuke Yumine, Natsuko Tsurudome, Masato Nishio, Machiko |
author_sort | Goto, Hideo |
collection | PubMed |
description | It has been reported that dual or multiple viruses can coinfect epithelial cells of the respiratory tract. However, little has been reported on in vitro interactions of coinfected viruses. To explore how coinfection of different viruses affects their biological property, we examined growth of influenza A virus (IAV) and human parainfluenza virus type 2 (hPIV2) during coinfection of Vero cells. We found that IAV growth was enhanced by coinfection with hPIV2. The enhanced growth of IAV was not reproduced by coinfection with an hPIV2 mutant with reduced cell fusion activity, or by ectopic expression of the V protein of hPIV2. In contrast, induction of cell fusion by ectopic expression of the hPIV2 HN and F proteins augments IAV growth. hPIV2 coinfection supported IAV growth in cells originated from the respiratory epithelium. The enhancement correlated closely with cell fusion ability of hPIV2 in those cells. These results indicate that cell fusion induced by hPIV2 infection is beneficial to IAV replication and that enhanced viral replication by coinfection with different viruses can modify their pathological consequences. |
format | Online Article Text |
id | pubmed-7086786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-70867862020-03-23 Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2 Goto, Hideo Ihira, Hironobu Morishita, Keiichi Tsuchiya, Mitsuki Ohta, Keisuke Yumine, Natsuko Tsurudome, Masato Nishio, Machiko Med Microbiol Immunol Original Investigation It has been reported that dual or multiple viruses can coinfect epithelial cells of the respiratory tract. However, little has been reported on in vitro interactions of coinfected viruses. To explore how coinfection of different viruses affects their biological property, we examined growth of influenza A virus (IAV) and human parainfluenza virus type 2 (hPIV2) during coinfection of Vero cells. We found that IAV growth was enhanced by coinfection with hPIV2. The enhanced growth of IAV was not reproduced by coinfection with an hPIV2 mutant with reduced cell fusion activity, or by ectopic expression of the V protein of hPIV2. In contrast, induction of cell fusion by ectopic expression of the hPIV2 HN and F proteins augments IAV growth. hPIV2 coinfection supported IAV growth in cells originated from the respiratory epithelium. The enhancement correlated closely with cell fusion ability of hPIV2 in those cells. These results indicate that cell fusion induced by hPIV2 infection is beneficial to IAV replication and that enhanced viral replication by coinfection with different viruses can modify their pathological consequences. Springer Berlin Heidelberg 2015-11-18 2016 /pmc/articles/PMC7086786/ /pubmed/26582554 http://dx.doi.org/10.1007/s00430-015-0441-y Text en © Springer-Verlag Berlin Heidelberg 2015 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Investigation Goto, Hideo Ihira, Hironobu Morishita, Keiichi Tsuchiya, Mitsuki Ohta, Keisuke Yumine, Natsuko Tsurudome, Masato Nishio, Machiko Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2 |
title | Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2 |
title_full | Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2 |
title_fullStr | Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2 |
title_full_unstemmed | Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2 |
title_short | Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2 |
title_sort | enhanced growth of influenza a virus by coinfection with human parainfluenza virus type 2 |
topic | Original Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086786/ https://www.ncbi.nlm.nih.gov/pubmed/26582554 http://dx.doi.org/10.1007/s00430-015-0441-y |
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