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Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains

A large number of accidental virus infections occur in medical and non-medical workers exposed to infectious individuals and materials. We evaluated whether postmortem blood and bloodstains containing hepatitis B virus (HBV) are infectious. HBV-infected blood and bloodstains were stored for up to 60...

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Autores principales: Hara, Junpei, Tanaka, Yuka, Kaneko, Hiroto, Itoh, Yoshito, Ikegaya, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086790/
https://www.ncbi.nlm.nih.gov/pubmed/29198036
http://dx.doi.org/10.1007/s00705-017-3665-x
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author Hara, Junpei
Tanaka, Yuka
Kaneko, Hiroto
Itoh, Yoshito
Ikegaya, Hiroshi
author_facet Hara, Junpei
Tanaka, Yuka
Kaneko, Hiroto
Itoh, Yoshito
Ikegaya, Hiroshi
author_sort Hara, Junpei
collection PubMed
description A large number of accidental virus infections occur in medical and non-medical workers exposed to infectious individuals and materials. We evaluated whether postmortem blood and bloodstains containing hepatitis B virus (HBV) are infectious. HBV-infected blood and bloodstains were stored for up to 60 days at room temperature and subsequently screened for hepatitis B surface antigen (HBsAg) and HBV DNA. In addition, HBV-positive postmortem blood was added to a cell line and the production of HBV virions was examined over a period of 7 days. HBsAg and HBV DNA were detected in all samples stored for 60 days at room temperature. HBV-positive postmortem blood successfully infected the cell line and progeny viruses were produced for up to 6 days. Thus, it is crucial that due care is taken when handling not only living material infected with HBV, as well as other harmful viruses, but also blood or body fluids from cadavers or medical waste.
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spelling pubmed-70867902020-03-23 Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains Hara, Junpei Tanaka, Yuka Kaneko, Hiroto Itoh, Yoshito Ikegaya, Hiroshi Arch Virol Original Article A large number of accidental virus infections occur in medical and non-medical workers exposed to infectious individuals and materials. We evaluated whether postmortem blood and bloodstains containing hepatitis B virus (HBV) are infectious. HBV-infected blood and bloodstains were stored for up to 60 days at room temperature and subsequently screened for hepatitis B surface antigen (HBsAg) and HBV DNA. In addition, HBV-positive postmortem blood was added to a cell line and the production of HBV virions was examined over a period of 7 days. HBsAg and HBV DNA were detected in all samples stored for 60 days at room temperature. HBV-positive postmortem blood successfully infected the cell line and progeny viruses were produced for up to 6 days. Thus, it is crucial that due care is taken when handling not only living material infected with HBV, as well as other harmful viruses, but also blood or body fluids from cadavers or medical waste. Springer Vienna 2017-12-02 2018 /pmc/articles/PMC7086790/ /pubmed/29198036 http://dx.doi.org/10.1007/s00705-017-3665-x Text en © Springer-Verlag GmbH Austria, part of Springer Nature 2017 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Hara, Junpei
Tanaka, Yuka
Kaneko, Hiroto
Itoh, Yoshito
Ikegaya, Hiroshi
Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains
title Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains
title_full Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains
title_fullStr Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains
title_full_unstemmed Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains
title_short Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains
title_sort detection of hepatitis b virus dna and hbsag from postmortem blood and bloodstains
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086790/
https://www.ncbi.nlm.nih.gov/pubmed/29198036
http://dx.doi.org/10.1007/s00705-017-3665-x
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