Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces

Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal sampl...

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Autores principales: Ellens, D. J., de Leeuw, P. W., Straver, P. J., van Balken, J. A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1978
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086822/
https://www.ncbi.nlm.nih.gov/pubmed/214679
http://dx.doi.org/10.1007/BF02121145
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author Ellens, D. J.
de Leeuw, P. W.
Straver, P. J.
van Balken, J. A. M.
author_facet Ellens, D. J.
de Leeuw, P. W.
Straver, P. J.
van Balken, J. A. M.
author_sort Ellens, D. J.
collection PubMed
description Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. Immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. The enzyme-linked immunosorbent assay demonstrated rotavirus antigens in 49 faecal samples. The assay is not only sensitive but also simple to perform and suitable for large-scale testing.
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spelling pubmed-70868222020-03-23 Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces Ellens, D. J. de Leeuw, P. W. Straver, P. J. van Balken, J. A. M. Med Microbiol Immunol Article Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. Immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. The enzyme-linked immunosorbent assay demonstrated rotavirus antigens in 49 faecal samples. The assay is not only sensitive but also simple to perform and suitable for large-scale testing. Springer-Verlag 1978 /pmc/articles/PMC7086822/ /pubmed/214679 http://dx.doi.org/10.1007/BF02121145 Text en © Springer-Verlag 1978 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Ellens, D. J.
de Leeuw, P. W.
Straver, P. J.
van Balken, J. A. M.
Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces
title Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces
title_full Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces
title_fullStr Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces
title_full_unstemmed Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces
title_short Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces
title_sort comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086822/
https://www.ncbi.nlm.nih.gov/pubmed/214679
http://dx.doi.org/10.1007/BF02121145
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