Susceptibility of different eukaryotic cell lines to SARS-coronavirus

In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS)...

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Detalles Bibliográficos
Autores principales: Hattermann, K., Müller, M. A., Nitsche, A., Wendt, S., Donoso Mantke, O., Niedrig, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2005
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086824/
https://www.ncbi.nlm.nih.gov/pubmed/15645376
http://dx.doi.org/10.1007/s00705-004-0461-1
Descripción
Sumario:In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.

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