Cargando…
Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus
The kinetics of appearance of antibodies directed to the major structural proteins N, M and E of porcine reproductive and respiratory syndrome virus (PRRSV) was followed in pigs naturally- and experimentally-exposed to the virus. Specific IgM antibody titers were first detected by indirect immunoflu...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
1996
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086943/ https://www.ncbi.nlm.nih.gov/pubmed/8645111 http://dx.doi.org/10.1007/BF01718333 |
_version_ | 1783509231716007936 |
---|---|
author | Loemba, H. D. Mounir, S. Mardassi, H. Archambault, D. Dea, S. |
author_facet | Loemba, H. D. Mounir, S. Mardassi, H. Archambault, D. Dea, S. |
author_sort | Loemba, H. D. |
collection | PubMed |
description | The kinetics of appearance of antibodies directed to the major structural proteins N, M and E of porcine reproductive and respiratory syndrome virus (PRRSV) was followed in pigs naturally- and experimentally-exposed to the virus. Specific IgM antibody titers were first detected by indirect immunofluorescence (IIF) at the end of the first week of PRRSV infection, peaked by day 14 to 21 post-inoculation (p.i.), then rapidly decreased to undetectable levels by day 35 to 42 p.i. On the other hand, specific IgG antibody titers peaked by day 21 to 28 p.i. and remained unchanged to the end of the 6- or 9-week observation period; in addition, a persistent viremia was observed. Virus neutralizing (VN) antibody titers >8 were not detected until 3 to 4 weeks p.i. Taken together, the results obtained by Western blotting analyses using purified virus andE. coli-expressed ORFs 5 to 7 gene products, suggested that antibodies directed against the envelope E protein appear by day 7 p.i., whereas antibodies directed against the nucleocapsid N and membrane M proteins can only be detected by the end of the second week p.i. No correlation could be demonstrated between VN and IIF antibody titers, viremia, and viral protein specificities of circulating antibodies at various times p.i. |
format | Online Article Text |
id | pubmed-7086943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1996 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-70869432020-03-23 Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus Loemba, H. D. Mounir, S. Mardassi, H. Archambault, D. Dea, S. Arch Virol Brief Report The kinetics of appearance of antibodies directed to the major structural proteins N, M and E of porcine reproductive and respiratory syndrome virus (PRRSV) was followed in pigs naturally- and experimentally-exposed to the virus. Specific IgM antibody titers were first detected by indirect immunofluorescence (IIF) at the end of the first week of PRRSV infection, peaked by day 14 to 21 post-inoculation (p.i.), then rapidly decreased to undetectable levels by day 35 to 42 p.i. On the other hand, specific IgG antibody titers peaked by day 21 to 28 p.i. and remained unchanged to the end of the 6- or 9-week observation period; in addition, a persistent viremia was observed. Virus neutralizing (VN) antibody titers >8 were not detected until 3 to 4 weeks p.i. Taken together, the results obtained by Western blotting analyses using purified virus andE. coli-expressed ORFs 5 to 7 gene products, suggested that antibodies directed against the envelope E protein appear by day 7 p.i., whereas antibodies directed against the nucleocapsid N and membrane M proteins can only be detected by the end of the second week p.i. No correlation could be demonstrated between VN and IIF antibody titers, viremia, and viral protein specificities of circulating antibodies at various times p.i. Springer-Verlag 1996 /pmc/articles/PMC7086943/ /pubmed/8645111 http://dx.doi.org/10.1007/BF01718333 Text en © Springer-Verlag 1996 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Brief Report Loemba, H. D. Mounir, S. Mardassi, H. Archambault, D. Dea, S. Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
title | Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
title_full | Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
title_fullStr | Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
title_full_unstemmed | Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
title_short | Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
title_sort | kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086943/ https://www.ncbi.nlm.nih.gov/pubmed/8645111 http://dx.doi.org/10.1007/BF01718333 |
work_keys_str_mv | AT loembahd kineticsofhumoralimmuneresponsetothemajorstructuralproteinsoftheporcinereproductiveandrespiratorysyndromevirus AT mounirs kineticsofhumoralimmuneresponsetothemajorstructuralproteinsoftheporcinereproductiveandrespiratorysyndromevirus AT mardassih kineticsofhumoralimmuneresponsetothemajorstructuralproteinsoftheporcinereproductiveandrespiratorysyndromevirus AT archambaultd kineticsofhumoralimmuneresponsetothemajorstructuralproteinsoftheporcinereproductiveandrespiratorysyndromevirus AT deas kineticsofhumoralimmuneresponsetothemajorstructuralproteinsoftheporcinereproductiveandrespiratorysyndromevirus |