Identification and sequence determination of the capsid protein gene of feline calicivirus

We have determined 4380 bases of the sequence from a cDNA clone containing the 3′ end of feline calicivirus strain F9. We find four candidate open reading frames of which three are complete and comprise 245, 317 and 2012 nucleotides. The fourth continues toward the 5′ end. We have expressed the larg...

Descripción completa

Detalles Bibliográficos
Autores principales: Carter, M. J., Milton, I. D., Turner, P. C., Meanger, J., Bennett, M., Gaskell, R. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1992
Materias:
Original Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086951/
https://www.ncbi.nlm.nih.gov/pubmed/1731695
http://dx.doi.org/10.1007/BF01317185
_version_ 1783509233575133184
author Carter, M. J.
Milton, I. D.
Turner, P. C.
Meanger, J.
Bennett, M.
Gaskell, R. M.
author_facet Carter, M. J.
Milton, I. D.
Turner, P. C.
Meanger, J.
Bennett, M.
Gaskell, R. M.
author_sort Carter, M. J.
collection PubMed
description We have determined 4380 bases of the sequence from a cDNA clone containing the 3′ end of feline calicivirus strain F9. We find four candidate open reading frames of which three are complete and comprise 245, 317 and 2012 nucleotides. The fourth continues toward the 5′ end. We have expressed the largest complete open reading frame inE. coli. Sera raised to this antigen react specifically with the capsid protein and its intracellular precursor molecule. N-terminal sequence analysis of purified, mature capsid protein confirms this assignment and has identified the position at which precursor is cleaved.
format Online
Article
Text
id pubmed-7086951
institution National Center for Biotechnology Information
language English
publishDate 1992
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-70869512020-03-23 Identification and sequence determination of the capsid protein gene of feline calicivirus Carter, M. J. Milton, I. D. Turner, P. C. Meanger, J. Bennett, M. Gaskell, R. M. Arch Virol Original Papers We have determined 4380 bases of the sequence from a cDNA clone containing the 3′ end of feline calicivirus strain F9. We find four candidate open reading frames of which three are complete and comprise 245, 317 and 2012 nucleotides. The fourth continues toward the 5′ end. We have expressed the largest complete open reading frame inE. coli. Sera raised to this antigen react specifically with the capsid protein and its intracellular precursor molecule. N-terminal sequence analysis of purified, mature capsid protein confirms this assignment and has identified the position at which precursor is cleaved. Springer-Verlag 1992 /pmc/articles/PMC7086951/ /pubmed/1731695 http://dx.doi.org/10.1007/BF01317185 Text en © Springer-Verlag 1992 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Papers
Carter, M. J.
Milton, I. D.
Turner, P. C.
Meanger, J.
Bennett, M.
Gaskell, R. M.
Identification and sequence determination of the capsid protein gene of feline calicivirus
title Identification and sequence determination of the capsid protein gene of feline calicivirus
title_full Identification and sequence determination of the capsid protein gene of feline calicivirus
title_fullStr Identification and sequence determination of the capsid protein gene of feline calicivirus
title_full_unstemmed Identification and sequence determination of the capsid protein gene of feline calicivirus
title_short Identification and sequence determination of the capsid protein gene of feline calicivirus
title_sort identification and sequence determination of the capsid protein gene of feline calicivirus
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086951/
https://www.ncbi.nlm.nih.gov/pubmed/1731695
http://dx.doi.org/10.1007/BF01317185
work_keys_str_mv AT cartermj identificationandsequencedeterminationofthecapsidproteingeneoffelinecalicivirus
AT miltonid identificationandsequencedeterminationofthecapsidproteingeneoffelinecalicivirus
AT turnerpc identificationandsequencedeterminationofthecapsidproteingeneoffelinecalicivirus
AT meangerj identificationandsequencedeterminationofthecapsidproteingeneoffelinecalicivirus
AT bennettm identificationandsequencedeterminationofthecapsidproteingeneoffelinecalicivirus
AT gaskellrm identificationandsequencedeterminationofthecapsidproteingeneoffelinecalicivirus

Ejemplares similares