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Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation

The Smith strain of mouse cytomegalovirus (MCMV) was infectious for infant and mature DA strain laboratory rats as judged by development of neutralizing antibodies and specific spleen cell proliferation on stimulation with MCMV antigen. An i. p. inoculum of 10(6) PFU of MCMV was fatal for more than...

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Autores principales: Smith, C. B., Wei, L. S., Griffiths, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1986
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086953/
https://www.ncbi.nlm.nih.gov/pubmed/3015085
http://dx.doi.org/10.1007/BF01317379
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author Smith, C. B.
Wei, L. S.
Griffiths, Marie
author_facet Smith, C. B.
Wei, L. S.
Griffiths, Marie
author_sort Smith, C. B.
collection PubMed
description The Smith strain of mouse cytomegalovirus (MCMV) was infectious for infant and mature DA strain laboratory rats as judged by development of neutralizing antibodies and specific spleen cell proliferation on stimulation with MCMV antigen. An i. p. inoculum of 10(6) PFU of MCMV was fatal for more than two-thirds of infant mice (1–7 days of age), and disseminated viral infection was documented by isolation of virus from body organs. In contrast, weanling and adult rats did not become ill as a result of infection with a larger inoculum of 10(7) PFU. However, these older MCMV infected rats did show transient reversals of T helper/suppressor cell ratios and alterations of immune cell function as detected byin vitro spleen cell proliferation assays. Seven days after MCMV infection, there was a generalized increase in(3)H-thymidine incorporation by spleen cells in both resting (unstimulated) cultures and cultures exposed to mitogens (Con A, PHA, LPS) and to MCMV antigen. At 14 days, the spleen cell proliferation in the unstimulated cultures returned to normal but was depressed compared to controls in response to Con A. These observations show that laboratory rats are susceptible to MCMV infection and that assymptomatic infection may occur and cause transient alterations in lymphocyte subsets and in their reactivity to mitogens.
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spelling pubmed-70869532020-03-23 Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation Smith, C. B. Wei, L. S. Griffiths, Marie Arch Virol Original Papers The Smith strain of mouse cytomegalovirus (MCMV) was infectious for infant and mature DA strain laboratory rats as judged by development of neutralizing antibodies and specific spleen cell proliferation on stimulation with MCMV antigen. An i. p. inoculum of 10(6) PFU of MCMV was fatal for more than two-thirds of infant mice (1–7 days of age), and disseminated viral infection was documented by isolation of virus from body organs. In contrast, weanling and adult rats did not become ill as a result of infection with a larger inoculum of 10(7) PFU. However, these older MCMV infected rats did show transient reversals of T helper/suppressor cell ratios and alterations of immune cell function as detected byin vitro spleen cell proliferation assays. Seven days after MCMV infection, there was a generalized increase in(3)H-thymidine incorporation by spleen cells in both resting (unstimulated) cultures and cultures exposed to mitogens (Con A, PHA, LPS) and to MCMV antigen. At 14 days, the spleen cell proliferation in the unstimulated cultures returned to normal but was depressed compared to controls in response to Con A. These observations show that laboratory rats are susceptible to MCMV infection and that assymptomatic infection may occur and cause transient alterations in lymphocyte subsets and in their reactivity to mitogens. Springer-Verlag 1986 /pmc/articles/PMC7086953/ /pubmed/3015085 http://dx.doi.org/10.1007/BF01317379 Text en © Springer-Verlag 1986 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Papers
Smith, C. B.
Wei, L. S.
Griffiths, Marie
Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
title Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
title_full Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
title_fullStr Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
title_full_unstemmed Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
title_short Mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
title_sort mouse cytomegalovirus is infectious for rats and alters lymphocyte subsets and spleen cell proliferation
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086953/
https://www.ncbi.nlm.nih.gov/pubmed/3015085
http://dx.doi.org/10.1007/BF01317379
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