Cargando…

Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I

cDNA clones spanning the entire region of the peplomer (S) gene of feline infectious peritonitis virus (FIPV) type I strain KU-2 were obtained and their complete nucleotide sequences were determined. A long open reading frame (ORF) encoding 1464 amino acid residues was found in the gene, which was 1...

Descripción completa

Detalles Bibliográficos
Autores principales: Motokawa, K., Hohdatsu, T., Aizawa, C., Koyama, H., Hashimoto, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086962/
https://www.ncbi.nlm.nih.gov/pubmed/7733820
http://dx.doi.org/10.1007/BF01718424
_version_ 1783509236153581568
author Motokawa, K.
Hohdatsu, T.
Aizawa, C.
Koyama, H.
Hashimoto, H.
author_facet Motokawa, K.
Hohdatsu, T.
Aizawa, C.
Koyama, H.
Hashimoto, H.
author_sort Motokawa, K.
collection PubMed
description cDNA clones spanning the entire region of the peplomer (S) gene of feline infectious peritonitis virus (FIPV) type I strain KU-2 were obtained and their complete nucleotide sequences were determined. A long open reading frame (ORF) encoding 1464 amino acid residues was found in the gene, which was 12 residues longer than the ORF of the FIPV type II strain 79–1146. The sequences of FIPV type I and mainly FIPV type II were compared. The homologies at the N- (amino acid residues 1–693) and C- (residues 694–1464) terminal halves were 29.8 and 60.7%, respectively. This was much lower than that between FIPV type II and other antigenically related coronaviruses, such as transmissible gastroenteritis virus of swine and canine coronavirus. This supported the serological relatedness of the viruses and confirmed that the peplomer protein of FIPV type I has distinct structural features that differ from those of antigenically related viruses.
format Online
Article
Text
id pubmed-7086962
institution National Center for Biotechnology Information
language English
publishDate 1995
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-70869622020-03-23 Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I Motokawa, K. Hohdatsu, T. Aizawa, C. Koyama, H. Hashimoto, H. Arch Virol Original Papers cDNA clones spanning the entire region of the peplomer (S) gene of feline infectious peritonitis virus (FIPV) type I strain KU-2 were obtained and their complete nucleotide sequences were determined. A long open reading frame (ORF) encoding 1464 amino acid residues was found in the gene, which was 12 residues longer than the ORF of the FIPV type II strain 79–1146. The sequences of FIPV type I and mainly FIPV type II were compared. The homologies at the N- (amino acid residues 1–693) and C- (residues 694–1464) terminal halves were 29.8 and 60.7%, respectively. This was much lower than that between FIPV type II and other antigenically related coronaviruses, such as transmissible gastroenteritis virus of swine and canine coronavirus. This supported the serological relatedness of the viruses and confirmed that the peplomer protein of FIPV type I has distinct structural features that differ from those of antigenically related viruses. Springer-Verlag 1995 /pmc/articles/PMC7086962/ /pubmed/7733820 http://dx.doi.org/10.1007/BF01718424 Text en © Springer-Verlag 1995 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Papers
Motokawa, K.
Hohdatsu, T.
Aizawa, C.
Koyama, H.
Hashimoto, H.
Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I
title Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I
title_full Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I
title_fullStr Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I
title_full_unstemmed Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I
title_short Molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type I
title_sort molecular cloning and sequence determination of the peplomer protein gene of feline infectious peritonitis virus type i
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086962/
https://www.ncbi.nlm.nih.gov/pubmed/7733820
http://dx.doi.org/10.1007/BF01718424
work_keys_str_mv AT motokawak molecularcloningandsequencedeterminationofthepeplomerproteingeneoffelineinfectiousperitonitisvirustypei
AT hohdatsut molecularcloningandsequencedeterminationofthepeplomerproteingeneoffelineinfectiousperitonitisvirustypei
AT aizawac molecularcloningandsequencedeterminationofthepeplomerproteingeneoffelineinfectiousperitonitisvirustypei
AT koyamah molecularcloningandsequencedeterminationofthepeplomerproteingeneoffelineinfectiousperitonitisvirustypei
AT hashimotoh molecularcloningandsequencedeterminationofthepeplomerproteingeneoffelineinfectiousperitonitisvirustypei