Molecular epidemiology of porcine epidemic diarrhea virus in China

Since early 2006, porcine epidemic diarrhea virus (PEDV) has been reemerging in immunized swine herds. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. The entire ORF3 genes of 12 PEDV field strains and one vaccine strain were sequenced. The ORF3 genes of Chinese PEDV field...

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Autores principales: Chen, Jianfei, Wang, Chengbao, Shi, Hongyan, Qiu, Huaji, Liu, Shengwang, Chen, Xiaojin, Zhang, Zhibang, Feng, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2010
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086990/
https://www.ncbi.nlm.nih.gov/pubmed/20544234
http://dx.doi.org/10.1007/s00705-010-0720-2
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author Chen, Jianfei
Wang, Chengbao
Shi, Hongyan
Qiu, Huaji
Liu, Shengwang
Chen, Xiaojin
Zhang, Zhibang
Feng, Li
author_facet Chen, Jianfei
Wang, Chengbao
Shi, Hongyan
Qiu, Huaji
Liu, Shengwang
Chen, Xiaojin
Zhang, Zhibang
Feng, Li
author_sort Chen, Jianfei
collection PubMed
description Since early 2006, porcine epidemic diarrhea virus (PEDV) has been reemerging in immunized swine herds. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. The entire ORF3 genes of 12 PEDV field strains and one vaccine strain were sequenced. The ORF3 genes of Chinese PEDV field strains (excluding CH/GSJIII/07) contain a single 672- or 675-nucleotide (nt) ORF, which encodes a 223- or 224-aa-long peptide. However, the CV777 vaccine strain and CH/GSJIII/07 contain a 276-nt ORF because of a 49-nt deletion at nt 245–293. The Chinese PEDV field strains and PEDV reference strains are divided into three groups based on the phylogenetic relationship of their ORF3 genes. Chinese PEDV field strains (excluding CH/GSJIII/07) have a close phylogenetic relationship to Korean strains and are genetically different from the PEDV vaccine strains. However, CH/GSJIII/07 has a close phylogenetic relationship to two vaccine strains, suggesting that it might have evolved from a live vaccine strain. Chinese PEDV field strains (excluding CH/GSJIII/07) can be differentiated from PEDV vaccine strains by a nested RT-PCR method.
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spelling pubmed-70869902020-03-23 Molecular epidemiology of porcine epidemic diarrhea virus in China Chen, Jianfei Wang, Chengbao Shi, Hongyan Qiu, Huaji Liu, Shengwang Chen, Xiaojin Zhang, Zhibang Feng, Li Arch Virol Brief Report Since early 2006, porcine epidemic diarrhea virus (PEDV) has been reemerging in immunized swine herds. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. The entire ORF3 genes of 12 PEDV field strains and one vaccine strain were sequenced. The ORF3 genes of Chinese PEDV field strains (excluding CH/GSJIII/07) contain a single 672- or 675-nucleotide (nt) ORF, which encodes a 223- or 224-aa-long peptide. However, the CV777 vaccine strain and CH/GSJIII/07 contain a 276-nt ORF because of a 49-nt deletion at nt 245–293. The Chinese PEDV field strains and PEDV reference strains are divided into three groups based on the phylogenetic relationship of their ORF3 genes. Chinese PEDV field strains (excluding CH/GSJIII/07) have a close phylogenetic relationship to Korean strains and are genetically different from the PEDV vaccine strains. However, CH/GSJIII/07 has a close phylogenetic relationship to two vaccine strains, suggesting that it might have evolved from a live vaccine strain. Chinese PEDV field strains (excluding CH/GSJIII/07) can be differentiated from PEDV vaccine strains by a nested RT-PCR method. Springer Vienna 2010-06-11 2010 /pmc/articles/PMC7086990/ /pubmed/20544234 http://dx.doi.org/10.1007/s00705-010-0720-2 Text en © Springer-Verlag 2010 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Chen, Jianfei
Wang, Chengbao
Shi, Hongyan
Qiu, Huaji
Liu, Shengwang
Chen, Xiaojin
Zhang, Zhibang
Feng, Li
Molecular epidemiology of porcine epidemic diarrhea virus in China
title Molecular epidemiology of porcine epidemic diarrhea virus in China
title_full Molecular epidemiology of porcine epidemic diarrhea virus in China
title_fullStr Molecular epidemiology of porcine epidemic diarrhea virus in China
title_full_unstemmed Molecular epidemiology of porcine epidemic diarrhea virus in China
title_short Molecular epidemiology of porcine epidemic diarrhea virus in China
title_sort molecular epidemiology of porcine epidemic diarrhea virus in china
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086990/
https://www.ncbi.nlm.nih.gov/pubmed/20544234
http://dx.doi.org/10.1007/s00705-010-0720-2
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