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Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents

The growth, stability and seroprevalence in laboratory rodents of the two known strains of mouse adenovirus were compared. The FL strain of mouse adenovirus grew in both L 929 murine fibroblasts and in CMT-93 murine rectal carcinoma cells, whereas the K 87 strain grew only in CMT-93 cells. The bulk...

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Autores principales: Smith, A. L., Winograd, Deborah F., Burrage, T. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 1986
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086991/
https://www.ncbi.nlm.nih.gov/pubmed/3022678
http://dx.doi.org/10.1007/BF01314283
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author Smith, A. L.
Winograd, Deborah F.
Burrage, T. G.
author_facet Smith, A. L.
Winograd, Deborah F.
Burrage, T. G.
author_sort Smith, A. L.
collection PubMed
description The growth, stability and seroprevalence in laboratory rodents of the two known strains of mouse adenovirus were compared. The FL strain of mouse adenovirus grew in both L 929 murine fibroblasts and in CMT-93 murine rectal carcinoma cells, whereas the K 87 strain grew only in CMT-93 cells. The bulk of the FL progeny virus was released from the host cells. K 87 virus was largely cell-associated. Both virus strains were stable at 37° C in liquid medium. The K 87 strain was completely inactivated after 5–15 minutes at 56° C, whereas FL infectivity was still detected after two hours at this temperature. Both virus strains were stable in the dessicated state for 14 days, although FL viability was more dependent on the presence of protein in the virus diluent. Seroepidemiologic data suggest that viruses antigenically related to mouse adenovirus are more prevalent among laboratory rats than among laboratory mice and that the virus(es) infecting rats differ from those infecting mice. Results of retrospective serologic testing suggest an association between mouse adenovirus and an outbreak of disease in a mouse breeding colony.
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spelling pubmed-70869912020-03-23 Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents Smith, A. L. Winograd, Deborah F. Burrage, T. G. Arch Virol Original Papers The growth, stability and seroprevalence in laboratory rodents of the two known strains of mouse adenovirus were compared. The FL strain of mouse adenovirus grew in both L 929 murine fibroblasts and in CMT-93 murine rectal carcinoma cells, whereas the K 87 strain grew only in CMT-93 cells. The bulk of the FL progeny virus was released from the host cells. K 87 virus was largely cell-associated. Both virus strains were stable at 37° C in liquid medium. The K 87 strain was completely inactivated after 5–15 minutes at 56° C, whereas FL infectivity was still detected after two hours at this temperature. Both virus strains were stable in the dessicated state for 14 days, although FL viability was more dependent on the presence of protein in the virus diluent. Seroepidemiologic data suggest that viruses antigenically related to mouse adenovirus are more prevalent among laboratory rats than among laboratory mice and that the virus(es) infecting rats differ from those infecting mice. Results of retrospective serologic testing suggest an association between mouse adenovirus and an outbreak of disease in a mouse breeding colony. Springer-Verlag 1986 /pmc/articles/PMC7086991/ /pubmed/3022678 http://dx.doi.org/10.1007/BF01314283 Text en © Springer-Verlag 1986 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Papers
Smith, A. L.
Winograd, Deborah F.
Burrage, T. G.
Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents
title Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents
title_full Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents
title_fullStr Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents
title_full_unstemmed Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents
title_short Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents
title_sort comparative biological characterization of mouse adenovirus strains fl and k 87 and seroprevalence in laboratory rodents
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086991/
https://www.ncbi.nlm.nih.gov/pubmed/3022678
http://dx.doi.org/10.1007/BF01314283
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