pH-dependent aggregation and secretion of soluble monomeric influenza hemagglutinin

We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a...

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Detalles Bibliográficos
Autores principales: Vanlandschoot, P., Beirnaert, E., Grooten, J., Min Jou, W., Fiers, W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7086992/
https://www.ncbi.nlm.nih.gov/pubmed/9541609
http://dx.doi.org/10.1007/s007050050282
Descripción
Sumario:We previously reported the expression of soluble A/Victoria/3/75 (H3N2) hemagglutinin in insect cells and the molecular and immunological structure of an aggregated fraction, only observed in cell supernatant when expression was performed at low pH [23]. Here we report that besides this aggregated a monomeric and possibly a trimeric structure is detected in cell supernatant, irrespective of the pH of the medium. Evidence is presented that the aggregated fraction is generated out of monomeric HA0s molecules due to a low intracellular pH encountered during secretion.

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